Electrophysiological characterisation of iPSC-derived human β-like cells and an
Multielectrode array
SLC30A8
electrical activity
electrical coupling
electrophysiology
exocytosis
hiPSC-derived Beta-like cells
insulin secretion
Journal
bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187
Informations de publication
Date de publication:
18 Oct 2023
18 Oct 2023
Historique:
pubmed:
31
10
2023
medline:
31
10
2023
entrez:
31
10
2023
Statut:
epublish
Résumé
iPSC-derived human β-like cells (BLC) hold promise for both therapy and disease modelling, but their generation remains challenging and their functional analyses beyond transcriptomic and morphological assessments remain limited. Here, we validate an approach using multicellular and single cell electrophysiological tools to evaluate BLCs functions. The Multi-Electrode Arrays (MEAs) measuring the extracellular electrical activity revealed that BLCs are electrically coupled, produce slow potential (SP) signals like primary β-cells that are closely linked to insulin secretion. We also used high-resolution single-cell patch-clamp measurements to capture the exocytotic properties, and characterize voltage-gated sodium and calcium currents. These were comparable to those in primary β and EndoC-βH1 cells. The K
Identifiants
pubmed: 37905040
doi: 10.1101/2023.10.17.561014
pmc: PMC10614917
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIDDK NIH HHS
ID : UM1 DK126185
Pays : United States
Références
J Physiol. 1967 Sep;192(2):407-36
pubmed: 4383089
Islets. 2016 Apr 18;8(3):83-95
pubmed: 27246810
Nat Genet. 2014 Apr;46(4):357-63
pubmed: 24584071
Physiol Rev. 2018 Jan 1;98(1):117-214
pubmed: 29212789
Biosens Bioelectron. 2018 Oct 15;117:253-259
pubmed: 29909196
Cell Rep Med. 2021 Apr 20;2(4):100238
pubmed: 33948571
Cell Calcium. 2017 Dec;68:45-61
pubmed: 29129207
Diabetologia. 2018 Jul;61(7):1614-1622
pubmed: 29675560
J Diabetes Investig. 2018 Mar;9(2):270-273
pubmed: 28597607
Diabetologia. 2012 Feb;55(2):372-81
pubmed: 22038519
Cell Metab. 2020 May 5;31(5):1004-1016.e5
pubmed: 32375022
Diabetes. 2013 Oct;62(10):3514-22
pubmed: 23630303
Diabetologia. 2021 Mar;64(3):630-640
pubmed: 33404684
Nat Biotechnol. 2014 Nov;32(11):1121-33
pubmed: 25211370
J Biol Eng. 2017 Jul 3;11:21
pubmed: 28680477
Pflugers Arch. 1992 Jan;420(1):121-3
pubmed: 1553260
Cell. 2014 Oct 9;159(2):428-39
pubmed: 25303535
J Clin Invest. 2011 Sep;121(9):3589-97
pubmed: 21865645
Diabetes. 2021 Apr;70(4):878-888
pubmed: 33468514
Cells. 2021 Jan 19;10(1):
pubmed: 33477961
Diabetes. 2000 Nov;49(11):1751-60
pubmed: 11078440
Lab Chip. 2015 Oct 7;15(19):3880-90
pubmed: 26282013
Nat Biotechnol. 2022 Jul;40(7):1042-1055
pubmed: 35241836
Diabetes. 2008 Jun;57(6):1618-28
pubmed: 18390794
Sci Rep. 2018 Nov 19;8(1):16994
pubmed: 30451893
Nat Commun. 2016 Jan 06;7:10080
pubmed: 26733021
Nat Genet. 2019 Nov;51(11):1596-1606
pubmed: 31676859
Diabetes Obes Metab. 2007 Nov;9 Suppl 2:118-32
pubmed: 17919186
J Mol Biol. 2020 Mar 6;432(5):1551-1578
pubmed: 31945378
Cell Rep. 2020 May 12;31(6):107623
pubmed: 32402282
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4505-9
pubmed: 2543980
J Physiol. 2012 Mar 1;590(5):1085-91
pubmed: 22199167
Ann N Y Acad Sci. 2009 Jan;1152:187-93
pubmed: 19161389
EMBO J. 2015 Jul 2;34(13):1759-72
pubmed: 25908839
Endocrinology. 2000 Jan;141(1):111-7
pubmed: 10614629
Annu Rev Physiol. 2013;75:155-79
pubmed: 22974438
Mol Metab. 2019 Mar;21:1-12
pubmed: 30630689
Diabetes. 2009 Feb;58(2):403-11
pubmed: 18984743
Eur J Endocrinol. 2021 Mar;184(3):455-468
pubmed: 33486469
Diabetologia. 2015 Jun;58(6):1291-9
pubmed: 25788295
Nat Rev Endocrinol. 2020 Apr;16(4):202-212
pubmed: 32099086
Cell Metab. 2018 Jan 9;27(1):57-67
pubmed: 28889951
Nat Commun. 2022 Jul 16;13(1):4142
pubmed: 35842441
Elife. 2018 Nov 09;7:
pubmed: 30412052
PLoS Comput Biol. 2016 Sep 28;12(9):e1005116
pubmed: 27681078