Effects of squamous cell carcinoma and smoking status on oropharyngeal and laryngeal microbial communities.
head and neck cancer
human papillomavirus
laryngeal squamous cell carcinoma
microbiota
oropharyngeal squamous cell carcinoma
smoking
Journal
Head & neck
ISSN: 1097-0347
Titre abrégé: Head Neck
Pays: United States
ID NLM: 8902541
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
07
10
2023
received:
04
08
2023
accepted:
16
10
2023
pubmed:
31
10
2023
medline:
31
10
2023
entrez:
31
10
2023
Statut:
ppublish
Résumé
Still, little is known about microbial dysbiosis in oropharyngeal and laryngeal tissue as risk factor for development of local squamous cell carcinoma. The site-specific microbiota at these regions in healthy and cancer tissue and their modulation by environmental factors need to be defined. The local microbiota of cancer tissue and healthy controls was profiled by 16S rRNA gene amplicon sequencing and statistical analysis using 111 oropharyngeal and 72 laryngeal intraoperative swabs. Oropharynx and larynx harbor distinct microbial communities. Clear effects of both smoking and cancer were seen in the oropharynx whereas effects in the larynx were minor. The distinct microbial communities at larynx and oropharynx partially explain why the effects of cancer and smoking were distinct at those sites. Thus, the use of microbiota supposed to mirror community changes in another target location should be avoided and more studies on the actual cancerous environment are necessary.
Sections du résumé
BACKGROUND
BACKGROUND
Still, little is known about microbial dysbiosis in oropharyngeal and laryngeal tissue as risk factor for development of local squamous cell carcinoma. The site-specific microbiota at these regions in healthy and cancer tissue and their modulation by environmental factors need to be defined.
METHODS
METHODS
The local microbiota of cancer tissue and healthy controls was profiled by 16S rRNA gene amplicon sequencing and statistical analysis using 111 oropharyngeal and 72 laryngeal intraoperative swabs.
RESULTS
RESULTS
Oropharynx and larynx harbor distinct microbial communities. Clear effects of both smoking and cancer were seen in the oropharynx whereas effects in the larynx were minor.
CONCLUSION
CONCLUSIONS
The distinct microbial communities at larynx and oropharynx partially explain why the effects of cancer and smoking were distinct at those sites. Thus, the use of microbiota supposed to mirror community changes in another target location should be avoided and more studies on the actual cancerous environment are necessary.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-160Informations de copyright
© 2023 The Authors. Head & Neck published by Wiley Periodicals LLC.
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