Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis.
coronavirus
double-membrane vesicle
host-pathogen interactions
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
30 Nov 2023
30 Nov 2023
Historique:
medline:
1
12
2023
pubmed:
31
10
2023
entrez:
31
10
2023
Statut:
ppublish
Résumé
Remodeling of the cellular endomembrane system by viruses allows for efficient and coordinated replication of the viral genome in distinct subcellular compartments termed replication organelles. As a critical step in the viral life cycle, replication organelle formation is an attractive target for therapeutic intervention, but factors central to this process are only partially understood. In this study, we corroborate that two viral proteins, nsp3 and nsp4, are the major drivers of membrane remodeling in SARS-CoV-2 infection. We further report a number of host cell factors interacting with these viral proteins and supporting the viral replication cycle, some of them by contributing to the formation of the SARS-CoV-2 replication organelle.
Identifiants
pubmed: 37905840
doi: 10.1128/jvi.00878-23
pmc: PMC10688318
doi:
Substances chimiques
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0087823Subventions
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : 240245660-SFB1129, 272983813-TRR 179
Organisme : Helmholtz-Fonds (Helmholtz-Fonds e.V.)
ID : KA1-Co-02 'COVIPA'
Organisme : European Molecular Biology Organization (EMBO)
ID : ALTF 454-2020
Organisme : Free and Hanseatic City of Hamburg
Organisme : Deutsche Forschungsgemeinschaft (DFG)
ID : SC 314/1-1
Organisme : Bundesministerium für Bildung und Forschung (BMBF)
ID : VirMScan
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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