Molecular basis for inhibition of type III-B CRISPR-Cas by an archaeal viral anti-CRISPR protein.

Archaeal Viruses / metabolism Viral Proteins / genetics CRISPR-Cas Systems Archaea / metabolism CRISPR-Associated Proteins / genetics
Acr inhibition mechanism CRISPR-Cas turnover anti-CRISPR type III CRISPR

Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
08 11 2023
Historique:
received: 23 02 2023
revised: 03 06 2023
accepted: 04 10 2023
medline: 13 11 2023
pubmed: 1 11 2023
entrez: 1 11 2023
Statut: ppublish

Résumé

Despite a wide presence of type III clustered regularly interspaced short palindromic repeats, CRISPR-associated (CRISPR-Cas) in archaea and bacteria, very few anti-CRISPR (Acr) proteins inhibiting type III immunity have been identified, and even less is known about their inhibition mechanism. Here, we present the discovery of a type III CRISPR-Cas inhibitor, AcrIIIB2, encoded by Sulfolobus virus S. islandicus rod-shaped virus 3 (SIRV3). AcrIIIB2 inhibits type III-B CRISPR-Cas immune response to protospacers encoded in middle/late-expressed viral genes. Investigation of the interactions between S. islandicus type III-B CRISPR-Cas Cmr-α-related proteins and AcrIIIB2 reveals that the Acr does not bind to Csx1 but rather interacts with the Cmr-α effector complex. Furthermore, in vitro assays demonstrate that AcrIIIB2 can block the dissociation of cleaved target RNA from the Cmr-α complex, thereby inhibiting the Cmr-α turnover, thus preventing host cellular dormancy and further viral genome degradation by the type III-B CRISPR-Cas immunity.

Identifiants

DOI: 10.1016/j.chom.2023.10.003 PMID: 37909049
pubmed: 37909049
pii: S1931-3128(23)00407-9
doi: 10.1016/j.chom.2023.10.003
pii:
doi:

Substances chimiques

Viral Proteins 0
CRISPR-Associated Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1837-1849.e5

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Jinzhong Lin (J)

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark.

Lauge Alfastsen (L)

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark.

Yuvaraj Bhoobalan-Chitty (Y)

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark.

Xu Peng (X)

Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark. Electronic address: peng@bio.ku.dk.

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Classifications MeSH

questionsmedicales.fr › Virus › Virus archéaux › Molecular basis for inhibition of type III-B...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Molecular basis for inhibition of type III-B...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Molecular basis for inhibition of type III-B...
questionsmedicales.fr › Archéobactéries › Molecular basis for inhibition of type III-B...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › Molecular basis for inhibition of type III-B...