Sulfonium-Cross-Linked Hyaluronic Acid-Based Self-Healing Hydrogel: Stimuli-Responsive Drug Carrier with Inherent Antibacterial Activity to Counteract Antibiotic-Resistant Bacteria.

antibacterial activity antibiofilm activity in vivo efficacy methicillin-resistant Staphylococcus aureus sulfonium-containing hyaluronic acid-based cross-linked hydrogel vancomycin-resistant Enterococci

Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
01 Nov 2023
Historique:
revised: 22 10 2023
received: 23 08 2023
pubmed: 1 11 2023
medline: 1 11 2023
entrez: 1 11 2023
Statut: aheadofprint

Résumé

Augmentation of the activity of Food and Drug Administration-approved antibiotics by an adjuvant or antibiotic carrier is considered one of the promising strategies to fight against antibiotic-resistant bacteria. This study reports the development of sulfonium-cross-linked hyaluronic acid (HA)-based polymer (HA-SS-HA) as an inherent antimicrobial agent and antibiotic carrier. The HA-SS-HA polymer offers the potential for encapsulating various classes of antibiotics and accomplishing a stimuli-responsive release profile in the presence of hyaluronidase produced by bacterial cells within their extracellular environment. Systematic antibacterial studies reveal that the HA-SS-HA-encapsulated antibiotics (vancomycin, amoxicillin, and tetracycline) restore its activity against the antibiotic-resistant bacterial cells methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE), and Pseudomonas aeruginosa. The HA-SS-HA gel shows robust efficacy in eradicating the mature biofilm of Staphylococcus aureus (S. aureus). The membrane-disrupting activity reveals that HA-SS-HA can also counteract the antibiotic resistance mechanism of the bacterial cells. The in vivo studies reveal excellent wound-healing activity of HA-SS-HA in albino laboratory-bred (BALB/c) mice. The outcome of additional antibacterial studies reveals that antibiotics-encapsulated HA-SS-HA hydrogel can effectively combat Gram-negative, Gram-positive, and antibiotic-resistant bacterial strains. Therefore, revitalizing the activity of commercial antibiotics by HA-SS-HA can be considered a valuable and economically effective strategy to fight against antibiotic-resistant bacteria.

Identifiants

pubmed: 37909063
doi: 10.1002/adhm.202302790
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2302790

Subventions

Organisme : Science & Engineering Research Board
ID : CRG/2021/000306
Organisme : Council of Scientific and Industrial Research, Government of India
ID : 02(420)/21/EMR-II

Informations de copyright

© 2023 Wiley-VCH GmbH.

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Auteurs

Anjali Patel (A)

Centre for Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Sanghamitra Goswami (S)

Department of Molecular Biology and Bioinformatics, Tripura University (A Central University), Agartala, Tripura, 799022, India.

Gunanka Hazarika (G)

Centre for Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Senthilkumar Sivaprakasam (S)

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Surajit Bhattacharjee (S)

Department of Molecular Biology and Bioinformatics, Tripura University (A Central University), Agartala, Tripura, 799022, India.

Debasis Manna (D)

Centre for Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Classifications MeSH