A Conserved Domain of HCV E2 Glycoprotein Interacts with Human CD81 and Induces Interferon-Gamma Secretion from Peripheral Blood Mononuclear Cells.
E2 glycoprotein
conserved domain
hCD81
hepatitis C virus
immune response
interferon-gamma
protein–protein interaction
Journal
Frontiers in bioscience (Landmark edition)
ISSN: 2768-6698
Titre abrégé: Front Biosci (Landmark Ed)
Pays: Singapore
ID NLM: 101612996
Informations de publication
Date de publication:
18 10 2023
18 10 2023
Historique:
received:
02
12
2022
revised:
15
04
2023
accepted:
24
05
2023
medline:
6
11
2023
pubmed:
3
11
2023
entrez:
2
11
2023
Statut:
ppublish
Résumé
Hepatitis C virus (HCV) infection is a global health threat to the public, and vaccines against it are not yet available. The HCV envelope glycoprotein E2 is a key target for anti-HCV vaccines. The majority of previous studies have focused on the hypervariable region and the glycosylation sites of the_HCV structural protein. This study aims to investigate a conserved domain of HCV E2 glycoprotein and explore its potential to induce an immune response against HCV. HCV E2 conserved domain (encompassing amino acids 505-702) was prepared in HCV E2 conserved domain was highly conserved among 25 HCV subtypes, and its recombinant soluble production in HCV E2 conserved domain directly binds hCD81 and activates the production of IFN-γ in the PBMCs of patients with HCV. Therefore, the conserved domain of HCV E2 glycoprotein may be a new candidate for developing an HCV vaccine.
Sections du résumé
BACKGROUND
Hepatitis C virus (HCV) infection is a global health threat to the public, and vaccines against it are not yet available. The HCV envelope glycoprotein E2 is a key target for anti-HCV vaccines. The majority of previous studies have focused on the hypervariable region and the glycosylation sites of the_HCV structural protein. This study aims to investigate a conserved domain of HCV E2 glycoprotein and explore its potential to induce an immune response against HCV.
METHODS
HCV E2 conserved domain (encompassing amino acids 505-702) was prepared in
RESULTS
HCV E2 conserved domain was highly conserved among 25 HCV subtypes, and its recombinant soluble production in
CONCLUSIONS
HCV E2 conserved domain directly binds hCD81 and activates the production of IFN-γ in the PBMCs of patients with HCV. Therefore, the conserved domain of HCV E2 glycoprotein may be a new candidate for developing an HCV vaccine.
Identifiants
pubmed: 37919063
pii: S2768-6701(23)00905-X
doi: 10.31083/j.fbl2810239
doi:
Substances chimiques
CD81 protein, human
0
Interferon-gamma
82115-62-6
Tetraspanin 28
0
Vaccines
0
IFNG protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
239Informations de copyright
© 2023 The Author(s). Published by IMR Press.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.