Process Analysis and Parameter Selection of Cardiomyocyte Electroporation Based on the Finite Element Method.
Cardiomyocyte
Finite element method
Pulsed-field ablation
Journal
Cardiovascular engineering and technology
ISSN: 1869-4098
Titre abrégé: Cardiovasc Eng Technol
Pays: United States
ID NLM: 101531846
Informations de publication
Date de publication:
02 2024
02 2024
Historique:
received:
07
06
2023
accepted:
18
10
2023
medline:
26
2
2024
pubmed:
3
11
2023
entrez:
3
11
2023
Statut:
ppublish
Résumé
Pulsed-field ablation (PFA) has attracted attention for the treatment of atrial fibrillation. This study aimed to further explore the relationship between the transmembrane voltage, pore radius and the intensity and duration of pulsed electric fields, which are closely related to the formation of irreversible electroporation. The different mechanisms of microsecond and nanosecond pulses acting on cardiomyocyte cellular and nuclear membranes were studied. A 3-D cardiomyocyte model with a nucleus was constructed to simulate the process of electroporation in cells under an electric field. Cell membrane electroporation was used to simulate the effect of different pulse parameters on the process of electroporation. Under a single pulse with a field strength of 1 kV/cm and width of 100 μs, the transmembrane potential (TMP) of the cell membrane reached 1.33 V, and the pore density and conductivity increased rapidly. The maximum pore radius of the cell membrane was 43.4 nm, and the electroporation area accounted for 4.6% of the total cell membrane area. The number of pores was positively correlated with the electric field intensity when the cell was exposed to electric fields of 0.5 to 6 kV/cm. Under a nanosecond pulse, the TMP of the nuclear and cell membranes exceeded 1 V after exposure to electric fields with strengths of 4 and 5 kV/cm, respectively. This study simulated the electroporation process of cardiomyocyte, and provides a basis for the selection of parameters for the application of PFA for application toward arrhythmias.
Identifiants
pubmed: 37919538
doi: 10.1007/s13239-023-00694-y
pii: 10.1007/s13239-023-00694-y
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22-38Subventions
Organisme : the National Key Research and Development Program
ID : 2021YFC 2400203
Organisme : the Shanghai Municipal Science and Economic and Informatization Commission Project
ID : GYQJ-2018-2-05
Organisme : Medical Engineering Fund of Fudan University
ID : yg2021-38
Informations de copyright
© 2023. The Author(s) under exclusive licence to Biomedical Engineering Society.
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