The effects of annual cycle, source population, and body condition on leukocyte profile and immune challenge in a basal reptile, the tuatara (Sphenodon punctatus).
blood smears
immunity
leukocytes
lymphocytes
reptiles
stress
Journal
Journal of experimental zoology. Part A, Ecological and integrative physiology
ISSN: 2471-5646
Titre abrégé: J Exp Zool A Ecol Integr Physiol
Pays: United States
ID NLM: 101710204
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
revised:
23
09
2023
received:
25
06
2023
accepted:
28
09
2023
pubmed:
3
11
2023
medline:
3
11
2023
entrez:
3
11
2023
Statut:
ppublish
Résumé
Leukocyte profiles are broadly used to assess the health status of many species. Reference intervals, and an understanding of the factors that may influence these intervals, are necessary for adequate interpretation of leukograms. Using a data set that spans over three decades, we investigated variation in leukocyte profile in several populations of the evolutionarily unique reptile, the tuatara (Sphenodon punctatus). To do this, we first established reference intervals for each leukocyte type according to best practices. Next, we determined that source population and sampling date were the two most important predictors of leukocyte makeup. We found significant differences in the ratio of heterophils: lymphocytes (H:L) between populations, with tuatara on the more resource-stressed sampling island having a significantly higher ratio of H:L. Finally, we found that sampling location, sex, and life stage did not explain variation in the responses of tuatara to stimulation with Concanavalin A and lipopolysaccharide in both 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide and Griess assay experiments. Our results offer important insight into the function of leukocytes in reptiles.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
60-72Subventions
Organisme : Society for Research on Amphibians and Reptiles in New Zealand
Organisme : Victoria University of Wellington
Organisme : Fulbright Association
Informations de copyright
© 2023 The Authors. Journal of Experimental Zoology Part A: Ecological and Integrative Physiology published by Wiley Periodicals LLC.
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