Clathrin light chains CLCa and CLCb have non-redundant roles in epithelial lumen formation.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
received:
19
05
2023
revised:
14
10
2023
accepted:
16
10
2023
medline:
7
11
2023
pubmed:
6
11
2023
entrez:
3
11
2023
Statut:
epublish
Résumé
To identify functional differences between vertebrate clathrin light chains (CLCa or CLCb), phenotypes of mice lacking genes encoding either isoform were characterised. Mice without CLCa displayed 50% neonatal mortality, reduced body weight, reduced fertility, and ∼40% of aged females developed uterine pyometra. Mice lacking CLCb displayed a less severe weight reduction phenotype compared with those lacking CLCa and had no survival or reproductive system defects. Analysis of female mice lacking CLCa that developed pyometra revealed ectopic expression of epithelial differentiation markers (FOXA2 and K14) and a reduced number of endometrial glands, indicating defects in the lumenal epithelium. Defects in lumen formation and polarity of epithelial cysts derived from uterine or gut cell lines were also observed when either CLCa or CLCb were depleted, with more severe effects from CLCa depletion. In cysts, the CLC isoforms had different distributions relative to each other, although they converge in tissue. Together, these findings suggest differential and cooperative roles for CLC isoforms in epithelial lumen formation, with a dominant function for CLCa.
Identifiants
pubmed: 37923360
pii: 7/1/e202302175
doi: 10.26508/lsa.202302175
pmc: PMC10624596
pii:
doi:
Substances chimiques
Clathrin Light Chains
0
Protein Isoforms
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023 Chen et al.
Références
Cell. 1992 Mar 6;68(5):899-910
pubmed: 1547490
Nat Commun. 2019 Oct 31;10(1):4974
pubmed: 31672988
Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9816-21
pubmed: 27540116
J Biol Chem. 2005 Feb 18;280(7):6109-17
pubmed: 15533940
J Leukoc Biol. 2013 Oct;94(4):585-94
pubmed: 23543767
Nature. 1987 Mar 12-18;326(6109):203-5
pubmed: 2434865
Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):E1018-E1026
pubmed: 28049832
Neuron. 2011 Nov 17;72(4):587-601
pubmed: 22099461
Vet Clin North Am Small Anim Pract. 2018 Jul;48(4):639-661
pubmed: 29933767
FASEB J. 2014 Jan;28(1):230-43
pubmed: 24025729
Nature. 1987 Mar 12-18;326(6109):154-9
pubmed: 3821891
Int J Mol Sci. 2018 Oct 19;19(10):
pubmed: 30347708
Front Endocrinol (Lausanne). 2018 Nov 02;9:653
pubmed: 30450081
Science. 2011 Oct 14;334(6053):255-8
pubmed: 21998396
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15064-9
pubmed: 18806221
Biol Reprod. 2009 Dec;81(6):1182-8
pubmed: 19684334
Nat Rev Mol Cell Biol. 2018 May;19(5):313-326
pubmed: 29410531
Trends Cell Biol. 2020 Sep;30(9):705-719
pubmed: 32620516
Traffic. 2018 May 16;:
pubmed: 29766620
Nihon Sanka Fujinka Gakkai Zasshi. 1991 Nov;43(11):1539-45
pubmed: 1940551
Reprod Biomed Online. 2018 Feb;36(2):181-187
pubmed: 29233503
Nat Cell Biol. 2012 Dec;14(12):1235-43
pubmed: 23196841
J Small Anim Pract. 1970 Dec;11(12):759-78
pubmed: 4924122
EMBO J. 1998 Aug 10;17(5):1297-303
pubmed: 9482727
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23527-23538
pubmed: 32907943
Nature. 1981 Jan 29;289(5796):420-2
pubmed: 7464911
J Reprod Med. 2001 Nov;46(11):952-6
pubmed: 11762150
Endocr Rev. 2019 Oct 1;40(5):1424-1445
pubmed: 31074826
Gene Expr Patterns. 2004 Dec;5(2):193-208
pubmed: 15567715
J Cell Biol. 2018 Dec 3;217(12):4253-4266
pubmed: 30228161
Biol Reprod. 2010 Sep;83(3):396-403
pubmed: 20484741
J Cell Sci. 2019 May 15;132(10):
pubmed: 30975920
Curr Opin Neurobiol. 2016 Aug;39:17-23
pubmed: 27016897
Science. 1987 Apr 17;236(4799):320-4
pubmed: 3563513
Curr Opin Cell Biol. 2020 Aug;65:141-149
pubmed: 32836101
Nat Commun. 2014 May 23;5:3891
pubmed: 24852344
Elife. 2019 Jun 04;8:
pubmed: 31159924
Mol Biol Cell. 2006 Oct;17(10):4343-52
pubmed: 16870700
Curr Biol. 2012 Aug 7;22(15):1361-70
pubmed: 22704991
Histochem Cell Biol. 2008 Jun;129(6):705-33
pubmed: 18461349
Annu Rev Cell Dev Biol. 2012;28:309-36
pubmed: 22831640
Reprod Toxicol. 2012 Aug;34(1):22-30
pubmed: 22429997
Cells. 2021 Feb 20;10(2):
pubmed: 33672612
Cell. 1981 Mar;23(3):755-61
pubmed: 7226229
Front Immunol. 2018 Mar 02;9:208
pubmed: 29552006
J Biol Chem. 2005 Feb 18;280(7):6101-8
pubmed: 15533941
Oncogene. 2013 Jul 18;32(29):3477-82
pubmed: 22945641
J Immunol. 2018 Dec 15;201(12):3669-3676
pubmed: 30464051
Endocrinology. 2018 Apr 1;159(4):1897-1909
pubmed: 29546371
J Cell Biol. 1983 Jul;97(1):244-51
pubmed: 6190820
Front Endocrinol (Lausanne). 2017 Oct 24;8:274
pubmed: 29123501