A single-domain intrabody targeting the follicle-stimulating hormone receptor impacts FSH-induced G protein-dependent signalling.
FSH receptor
G protein-coupled receptors
cell signalling
intracellular VHH
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
03 Nov 2023
03 Nov 2023
Historique:
revised:
20
09
2023
received:
17
07
2023
accepted:
06
10
2023
medline:
6
11
2023
pubmed:
6
11
2023
entrez:
3
11
2023
Statut:
aheadofprint
Résumé
Intracellular variable fragments of heavy-chain antibody from camelids (intra-VHH) have been successfully used as chaperones to solve the 3D structure of active G protein-coupled receptors bound to their transducers. However, their effect on signalling has been poorly explored, although they may provide a better understanding of the relationships between receptor conformation and activity. Here, we isolated and characterized iPRC1, the first intra-VHH recognizing a member of the large glycoprotein hormone receptor family, the follicle-stimulating hormone receptor (FSHR). This intra-VHH recognizes the FSHR third intracellular loop and decreases cAMP production in response to FSH, without altering Gαs recruitment. Hence, iPRC1 behaves as an allosteric modulator and provides a new tool to complete structure/activity studies performed thus far on this receptor.
Identifiants
pubmed: 37923554
doi: 10.1002/1873-3468.14765
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Organisme : LabEx MAbImprove
ID : ANR-10-LABX-53
Organisme : Région Centre Val de Loire RD2020 Biomédicaments SELMAT
Organisme : Région Centre Val de Loire APR-IR
ID : INTACT
Organisme : Centre National de la Recherche Scientifique (CNRS)
Informations de copyright
© 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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