Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies.
Antimicrobial resistance
Arcobacter butzleri
Caco-2 infection
Comparative genomics
Virulence
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
04 Nov 2023
04 Nov 2023
Historique:
received:
23
08
2023
accepted:
13
10
2023
revised:
28
09
2023
medline:
6
11
2023
pubmed:
4
11
2023
entrez:
4
11
2023
Statut:
epublish
Résumé
The surge in human arcobacteriosis has increased interest in determining the mechanisms involved in the pathogenesis of Arcobacter butzleri. Here, genomic analyses and in vitro Caco-2 infection, motility, urease and antimicrobial susceptibility testing (AST) assays were used to characterise the virulence and antimicrobial resistance (AMR) determinants of strains HC-1, isolated from a patient with travellers' diarrhoea, and HC-2, isolated from another with pruritus. AMR determinants conferring resistance to tetracycline (tetO, present in both genomes) and to ampicillin and amoxicillin-clavulanic acid (bla3, present in HC-2) were identified. The same determinants associated with flagellum, chemotaxis, adhesion and invasion were detected in both, but HC-1 lacked eight flagellar genes. The urease cluster was only present in HC-1. Motility and urease tests confirmed the genetic differences between strains, but no genetic marker related to the inability of HC-2 to adhere and invade was identified. This inability could be conditioning the patient's pathology.
Identifiants
pubmed: 37923944
doi: 10.1007/s00203-023-03709-3
pii: 10.1007/s00203-023-03709-3
pmc: PMC10624747
doi:
Substances chimiques
Urease
EC 3.5.1.5
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
369Subventions
Organisme : Eusko Jaurlaritza
ID : PA20/03
Organisme : Euskal Herriko Unibertsitatea
ID : GIU21/021
Informations de copyright
© 2023. The Author(s).
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