Utilization of an aqueous two-phase emulsification to prepare bimodal porous cellulose monolith for efficient adsorption of bovine serum albumin.
Aqueous two-phase system
Bimodal porous structure, protein adsorption
Cellulose monolith
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
06 Dec 2023
06 Dec 2023
Historique:
received:
16
07
2023
revised:
17
10
2023
accepted:
19
10
2023
medline:
27
11
2023
pubmed:
6
11
2023
entrez:
5
11
2023
Statut:
ppublish
Résumé
Cellulose monolith has garnered significant interest in the field of biochromatography, which lies in its interconnected porous structure, large surface area and biocompatibility. In this context, we propose a novel approach for preparing cellulose monoliths using an aqueous two-phase system devoid of any organic solvents and surfactants. In this strategy, emulsifying cellulose solution into PEG 20,000 solution gives bicontinuous aqueous phases and further porous cellulose monolith after regeneration of dissolved cellulose. And the macroporous channels are derived from the removal of the PEG 20,000 aqueous phase while the micropores are from the phase separation of the cellulose phase. Physical characterizations reveal the obtained cellulose monolith exhibits exceptional column permeability of 1.36 × 10
Identifiants
pubmed: 37926008
pii: S0021-9673(23)00696-9
doi: 10.1016/j.chroma.2023.464471
pii:
doi:
Substances chimiques
Serum Albumin, Bovine
27432CM55Q
2-diethylaminoethanol
S6DL4M053U
Cellulose
9004-34-6
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
464471Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.