Dynamic Micropatterning Reveals Substrate-Dependent Differences in the Geometric Control of Cell Polarization and Migration.
cell migration
cell polarity
extracellular matrices
micropatterns
Journal
Small methods
ISSN: 2366-9608
Titre abrégé: Small Methods
Pays: Germany
ID NLM: 101724536
Informations de publication
Date de publication:
05 Nov 2023
05 Nov 2023
Historique:
revised:
03
10
2023
received:
08
06
2023
medline:
6
11
2023
pubmed:
6
11
2023
entrez:
5
11
2023
Statut:
aheadofprint
Résumé
Cells are highly dynamic and adopt variable shapes and sizes. These variations are biologically important but challenging to investigate in a spatiotemporally controlled manner. Micropatterning, confining cells on microfabricated substrates with defined geometries and molecular compositions, is a powerful tool for controlling cell shape and interactions. However, conventional binary micropatterns are static and fail to address dynamic changes in cell polarity, spreading, and migration. Here, a method for dynamic micropatterning is reported, where the non-adhesive surface surrounding adhesive micropatterns is rapidly converted to support specific cell-matrix interactions while allowing simultaneous imaging of the cells. The technique is based on ultraviolet photopatterning of biotinylated polyethylene glycol-grafted poly-L-lysine, and it is simple, inexpensive, and compatible with a wide range of streptavidin-conjugated ligands. Experiments using biotinylation-based dynamic micropatterns reveal that distinct extracellular matrix ligands and bivalent integrin-clustering antibodies support different degrees of front-rear polarity in human glioblastoma cells, which correlates to altered directionality and persistence upon release and migration on fibronectin. Unexpectedly, however, neither an asymmetric cell shape nor centrosome orientation can fully predict the future direction of migration. Taken together, biotinylation-based dynamic micropatterns allow easily accessible and highly customizable control over cell morphology and motility.
Identifiants
pubmed: 37926786
doi: 10.1002/smtd.202300719
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300719Subventions
Organisme : Finnish Cancer Institute
Organisme : Research Council of Finland's Centre of Excellence Program
ID : 346131
Organisme : Cancer Foundation Finland
Organisme : University of Turku Doctoral Programme in Molecular Life Sciences
Organisme : Turku Doctoral Programme of Molecular Medicine
Organisme : Finnish Cultural Foundation
Organisme : Orion Research Foundation
Organisme : K. Albin Johansson's Foundation
Organisme : Research Council of Finland
ID : 325464
Organisme : Research Council of Finland
ID : 25700
Organisme : Research Council of Finland
ID : 296684
Organisme : Research Council of Finland
ID : 307313
Organisme : Sigrid Jusélius Foundation
Organisme : Jane and Aatos Erkko Foundation
Organisme : Research Council of Finland's InFLAMES Flagship Programme
ID : 337530
Informations de copyright
© 2023 The Authors. Small Methods published by Wiley-VCH GmbH.
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