Peptide Conjugates of a 2'-


Journal

ACS omega
ISSN: 2470-1343
Titre abrégé: ACS Omega
Pays: United States
ID NLM: 101691658

Informations de publication

Date de publication:
31 Oct 2023
Historique:
received: 17 07 2023
accepted: 26 09 2023
medline: 6 11 2023
pubmed: 6 11 2023
entrez: 6 11 2023
Statut: epublish

Résumé

Antisense oligonucleotides (ASOs) are short, single-stranded nucleic acid molecules that alter gene expression. However, their transport into appropriate cellular compartments is a limiting factor in their potency. Here, we synthesized splice-switching oligonucleotides (SSOs) previously developed to treat the rare disease erythropoietic protoporphyria. Using chemical ligation-quantitative polymerase chain reaction (CL-qPCR), we quantified the SSOs in cells and subcellular compartments following free uptake. To drive nuclear localization, we covalently conjugated nuclear localization signal (NLS) peptides to a lead 2'-

Identifiants

pubmed: 37929104
doi: 10.1021/acsomega.3c05144
pmc: PMC10620785
doi:

Types de publication

Journal Article

Langues

eng

Pagination

40463-40481

Informations de copyright

© 2023 The Authors. Published by American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare no competing financial interest.

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Auteurs

Alyssa C Hill (AC)

Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Eidgenössische Technische Hochschule Zürich (ETH Zürich), Zürich 8093, Switzerland.

J Philipp Becker (JP)

Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Eidgenössische Technische Hochschule Zürich (ETH Zürich), Zürich 8093, Switzerland.

Daria Slominski (D)

Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Eidgenössische Technische Hochschule Zürich (ETH Zürich), Zürich 8093, Switzerland.

François Halloy (F)

Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Eidgenössische Technische Hochschule Zürich (ETH Zürich), Zürich 8093, Switzerland.

Christoffer Søndergaard (C)

Roche Innovation Center Copenhagen (RICC), Hørsholm 2970, Denmark.

Jacob Ravn (J)

Roche Innovation Center Copenhagen (RICC), Hørsholm 2970, Denmark.

Jonathan Hall (J)

Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, Eidgenössische Technische Hochschule Zürich (ETH Zürich), Zürich 8093, Switzerland.

Classifications MeSH