SARS-CoV-2 NSP12 associates with TRiC and the P323L substitution acts as a host adaption.
NSP12
P323L
SARS-CoV-2
TRiC
phosphatase
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
30 Nov 2023
30 Nov 2023
Historique:
medline:
1
12
2023
pubmed:
6
11
2023
entrez:
6
11
2023
Statut:
ppublish
Résumé
SARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability to partially evade either natural or vaccine-acquired immunity. Some of these viruses have been labeled Variants of Concern (VoCs). At the root of all VoCs are two mutations, one in the viral spike protein that has been very well characterized and the other in the virus polymerase (NSP12). This is the viral protein responsible for replicating the genome. We show that NSP12 associates with host cell proteins that act as a scaffold to facilitate the function of this protein. Furthermore, we found that different variants of NSP12 interact with host cell proteins in subtle and different ways, which affect function.
Identifiants
pubmed: 37929963
doi: 10.1128/jvi.00424-23
pmc: PMC10688337
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
NSP12 protein, SARS-CoV-2
EC 2.7.7.48
Coronavirus RNA-Dependent RNA Polymerase
EC 2.7.7.48
MARVELD2 protein, human
0
MARVEL Domain Containing 2 Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0042423Subventions
Organisme : Medical Research Council
ID : MR/V027506/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
J Virol. 2023 Feb 28;97(2):e0168822
pubmed: 36656012
J Virol. 2015 Jan 15;89(2):917-30
pubmed: 25355874
Nat Commun. 2019 May 28;10(1):2342
pubmed: 31138817
Nat Commun. 2021 Sep 28;12(1):5695
pubmed: 34584097
J Virol. 2013 Sep;87(17):9486-500
pubmed: 23637410
Curr Protoc Bioinformatics. 2015 Mar 09;49:8.19.1-8.19.16
pubmed: 25754993
Viruses. 2019 Oct 10;11(10):
pubmed: 31658630
Microbiol Spectr. 2022 Oct 26;10(5):e0121922
pubmed: 36069583
Nature. 2022 Feb;602(7897):487-495
pubmed: 34942634
Nat Commun. 2020 Nov 26;11(1):6013
pubmed: 33243994
Nature. 2021 Apr;592(7852):116-121
pubmed: 33106671
Science. 2020 Oct 30;370(6516):564-570
pubmed: 32912998
Cell Rep. 2014 Nov 6;9(3):955-66
pubmed: 25437552
J Virol. 2010 Sep;84(17):8691-9
pubmed: 20573828
J Proteome Res. 2019 Feb 1;18(2):623-632
pubmed: 30450911
Cell. 2015 Nov 19;163(5):1108-1123
pubmed: 26582131
Genome Biol. 2020 Sep 7;21(1):238
pubmed: 32894206
Science. 2020 Sep 11;369(6509):1395-1398
pubmed: 32763915
N Engl J Med. 2022 Apr 21;386(16):1532-1546
pubmed: 35249272
Cancer Cell. 2020 Jul 13;38(1):115-128.e9
pubmed: 32589942
J Virol. 2007 Sep;81(17):9601-4
pubmed: 17567688
J Proteomics. 2014 Apr 04;100:37-43
pubmed: 24513533
Genome Biol. 2023 Mar 13;24(1):47
pubmed: 36915185
Science. 2021 Feb 26;371(6532):926-931
pubmed: 33495306
Science. 2022 Aug 26;377(6609):951-959
pubmed: 35881010
Nat Commun. 2015 Jun 17;6:7491
pubmed: 26082035
Nat Commun. 2020 Jul 21;11(1):3656
pubmed: 32694517
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Nat Protoc. 2006;1(6):2856-60
pubmed: 17406544
J Gen Virol. 2021 Apr;102(4):
pubmed: 33855951
Sci Rep. 2021 Jul 1;11(1):13705
pubmed: 34210996
Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18590-5
pubmed: 16339318
Nature. 2021 Nov;599(7883):114-119
pubmed: 34488225
Nature. 2021 May;593(7858):266-269
pubmed: 33767447
Cell. 2020 Jul 23;182(2):417-428.e13
pubmed: 32526208
Nature. 2020 Jul;583(7816):459-468
pubmed: 32353859
EMBO J. 2020 Dec 1;39(23):e106267
pubmed: 33051876
Nat Methods. 2011 Jan;8(1):70-3
pubmed: 21131968