Cell Counting Techniques for Preimplantation Mouse Embryos Using Fluorescent DNA Dyes.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
06 Nov 2023
06 Nov 2023
Historique:
medline:
7
11
2023
pubmed:
7
11
2023
entrez:
6
11
2023
Statut:
aheadofprint
Résumé
Counting cells in preimplantation embryos by light microscopy is straightforward until morula compaction, when cell boundaries in living embryos become indistinct. An alternative to morphological assessment of cell number is to use fluorescent DNA dyes. This protocol details simple nuclear counting with a single DNA dye (Hoechst) or a more complicated procedure in which differential nuclear counts of the trophectoderm and inner cell mass (ICM) can be made using immunosurgery of the blastocyst and two DNA dyes (Hoechst and propidium iodide).
Identifiants
pubmed: 37932075
pii: pdb.prot108091
doi: 10.1101/pdb.prot108091
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023 Cold Spring Harbor Laboratory Press.