Optogenetics for light control of biological systems.
Journal
Nature reviews. Methods primers
ISSN: 2662-8449
Titre abrégé: Nat Rev Methods Primers
Pays: England
ID NLM: 101778010
Informations de publication
Date de publication:
2022
2022
Historique:
medline:
1
1
2022
pubmed:
1
1
2022
entrez:
7
11
2023
Statut:
ppublish
Résumé
Optogenetic techniques have been developed to allow control over the activity of selected cells within a highly heterogeneous tissue, using a combination of genetic engineering and light. Optogenetics employs natural and engineered photoreceptors, mostly of microbial origin, to be genetically introduced into the cells of interest. As a result, cells that are naturally light-insensitive can be made photosensitive and addressable by illumination and precisely controllable in time and space. The selectivity of expression and subcellular targeting in the host is enabled by applying control elements such as promoters, enhancers and specific targeting sequences to the employed photoreceptor-encoding DNA. This powerful approach allows precise characterization and manipulation of cellular functions and has motivated the development of advanced optical methods for patterned photostimulation. Optogenetics has revolutionized neuroscience during the past 15 years and is primed to have a similar impact in other fields, including cardiology, cell biology and plant sciences. In this Primer, we describe the principles of optogenetics, review the most commonly used optogenetic tools, illumination approaches and scientific applications and discuss the possibilities and limitations associated with optogenetic manipulations across a wide variety of optical techniques, cells, circuits and organisms.
Identifiants
pubmed: 37933248
doi: 10.1038/s43586-022-00136-4
pmc: PMC10627578
mid: NIHMS1941402
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL144157
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB026152
Pays : United States
Déclaration de conflit d'intérêts
Competing interests Z.-H.P. is a co-inventor on patents related to optogenetic vision restoration and is also a co-founder and scientific advisor of Ray Therapeutics. The other authors declare no competing interests.
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