Meconium concentrations of pesticides and risk of hypospadias: a case-control study in Brittany, France.


Journal

Epidemiology (Cambridge, Mass.)
ISSN: 1531-5487
Titre abrégé: Epidemiology
Pays: United States
ID NLM: 9009644

Informations de publication

Date de publication:
02 Nov 2023
Historique:
medline: 7 11 2023
pubmed: 7 11 2023
entrez: 7 11 2023
Statut: aheadofprint

Résumé

Hypospadias is a male genital tract defect for which an increase in prevalence has been documented over the last few decades. A role for environmental risk factors is suspected, including prenatal exposure to pesticides. To study risk of hypospadias in association with multiple pesticide measurements in meconium samples. The Brittany Registry of Congenital Anomalies (France) conducted a case-control study between 2012 and 2018. Cases were hypospadias, ascertained by a pediatrician and a pediatric surgeon, excluding genetic conditions, following EUROCAT guidelines (N=69). Controls (N=135) were two male infants without congenital anomaly born after each case in the same maternity unit. Mothers in the maternity units completed a self-administered questionnaire, we collected medical data from hospital records, and medical staff collected meconium samples. We performed chemical analysis of 38 pesticides (parent compound and/or metabolite) by UHPLC/MS/MS following strict quality assurance/quality control criteria and blind to case-control status. We carried out logistic regression accounting for frequency-matching variables and major risk factors. Among the 38 pesticides measured, 16 (42%) were never detected in the meconium samples, 18 (47%) were in <5% of samples, and 4 (11%) in ≥5% of the samples. We observed an association between detection of fenitrothion in meconium and risk of hypospadias (OR=2.6 [1.0-6.3] with ncases=13, ncontrols=21), but not the other pesticides. Our small study provides robust assessment of fetal exposure. Fenitrothion's established antiandrogenic activities provide biologic plausibility for our observations. Further studies are needed to confirm this hypothesis.

Sections du résumé

BACKGROUND BACKGROUND
Hypospadias is a male genital tract defect for which an increase in prevalence has been documented over the last few decades. A role for environmental risk factors is suspected, including prenatal exposure to pesticides.
OBJECTIVES OBJECTIVE
To study risk of hypospadias in association with multiple pesticide measurements in meconium samples.
METHODS METHODS
The Brittany Registry of Congenital Anomalies (France) conducted a case-control study between 2012 and 2018. Cases were hypospadias, ascertained by a pediatrician and a pediatric surgeon, excluding genetic conditions, following EUROCAT guidelines (N=69). Controls (N=135) were two male infants without congenital anomaly born after each case in the same maternity unit. Mothers in the maternity units completed a self-administered questionnaire, we collected medical data from hospital records, and medical staff collected meconium samples. We performed chemical analysis of 38 pesticides (parent compound and/or metabolite) by UHPLC/MS/MS following strict quality assurance/quality control criteria and blind to case-control status. We carried out logistic regression accounting for frequency-matching variables and major risk factors.
RESULTS RESULTS
Among the 38 pesticides measured, 16 (42%) were never detected in the meconium samples, 18 (47%) were in <5% of samples, and 4 (11%) in ≥5% of the samples. We observed an association between detection of fenitrothion in meconium and risk of hypospadias (OR=2.6 [1.0-6.3] with ncases=13, ncontrols=21), but not the other pesticides.
CONCLUSIONS CONCLUSIONS
Our small study provides robust assessment of fetal exposure. Fenitrothion's established antiandrogenic activities provide biologic plausibility for our observations. Further studies are needed to confirm this hypothesis.

Identifiants

pubmed: 37934147
doi: 10.1097/EDE.0000000000001688
pii: 00001648-990000000-00197
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2023 Wolters Kluwer Health, Inc. All rights reserved.

Auteurs

Florence Rouget (F)

Brittany Registry of Congenital Anomalies, CHU Rennes, Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Adèle Bihannic (A)

Brittany Registry of Congenital Anomalies, CHU Rennes, F-35000 Rennes, France.

Barbara Le Bot (B)

Univ Rennes, EHESP, Inserm, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Fabien Mercier (F)

Univ Rennes, EHESP, Inserm, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Erwann Gilles (E)

Univ Rennes, EHESP, Inserm, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Ronan Garlantezec (R)

CHU Rennes, Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Luc Multigner (L)

Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Sylvaine Cordier (S)

Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Alexis Arnaud (A)

Department of Pediatric Surgery, CHU Rennes, F-35000 Rennes, France.

Patrick Pladys (P)

Univ Rennes, CHU Rennes, Inserm, LTSI-UMR 1099, Rennes, France.

Cécile Chevrier (C)

Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, F-35000 Rennes, France.

Classifications MeSH