Biosynthesis of magnetosome-nanobody complex in Magnetospirillum gryphiswaldense MSR-1 and a magnetosome-nanobody-based enzyme-linked immunosorbent assay for the detection of tetrabromobisphenol A in water.

Environmental monitoring Enzyme-linked immunosorbent assay Magnetosome Magnetospirillum gryphiswaldense Nanobody Tetrabromobisphenol A

Journal

Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327

Informations de publication

Date de publication:
07 Nov 2023
Historique:
received: 19 08 2023
accepted: 13 10 2023
revised: 12 10 2023
medline: 7 11 2023
pubmed: 7 11 2023
entrez: 7 11 2023
Statut: aheadofprint

Résumé

In this study, two mutant strains, TBC and TBC+, able to biosynthesize a novel functional magnetosome-nanobody (Nb), were derived from the magnetotactic bacteria Magnetospirillum gryphiswaldense MSR-1. The magnetosome-Nbs biosynthesized by TBC+ containing multi-copies of the Nb gene had a higher binding ability to an environmental pollutant, tetrabromobisphenol A (TBBPA), than those biosynthesized by TBC containing only one copy of the Nb gene. The magnetosome-Nbs from TBC+ can effectively bind to TBBPA in solutions with high capacity without being affected by a broad range of NaCl and methanol concentrations as well as pH. Therefore, a magnetosome-Nb-based enzyme-linked immunosorbent assay (ELISA) was developed and optimized for the detection of TBBPA, yielding a half-maximum signal inhibition concentration of 0.23 ng/mL and a limit of detection of 0.025 ng/mL. The assay was used to detect TBBPA in spiked river water samples, giving average recoveries between 90 and 120% and coefficients of variation of 2.5-6.3%. The magnetosome-Nb complex could be reused 4 times in ELISA without affecting the performance of the assay. Our results demonstrate the potential of magnetosome-Nbs produced by TBC+ as cost-effective and environment-friendly reagents for immunoassays to detect small molecules in environmental waters.

Identifiants

DOI: 10.1007/s00216-023-05005-x PMID: 37934249
pubmed: 37934249
doi: 10.1007/s00216-023-05005-x
pii: 10.1007/s00216-023-05005-x
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Chinese Polar Environment Comprehensive Investigation and Assessment Programmes
ID : 2018YFC1602900
Organisme : National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program
ID : P42ES04699
Organisme : NIEHS RIVER Award
ID : R35ES030443
Organisme : USDA Hatch Project
ID : HAW05044-R
Organisme : Key Project of Inter-Governmental International Scientific and Technological Innovation Cooperation
ID : 2019YFE0115800

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.

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Auteurs

Sha Wu (S)

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.
Suzhou Vicheck Biotechnology Co. Ltd., Suzhou, 215128, China.

Jiesheng Tian (J)

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Xianle Xue (X)

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.
Suzhou Vicheck Biotechnology Co. Ltd., Suzhou, 215128, China.

Fengfei Ma (F)

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.
Suzhou Vicheck Biotechnology Co. Ltd., Suzhou, 215128, China.

Qing X Li (QX)

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI, 96822, USA.

Christophe Morisseau (C)

Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, CA, 95616, USA.

Bruce D Hammock (BD)

Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, CA, 95616, USA.

Ting Xu (T)

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China. xuting@cau.edu.cn.
Suzhou Vicheck Biotechnology Co. Ltd., Suzhou, 215128, China. xuting@cau.edu.cn.

Classifications MeSH