Protocol for isolating mice skeletal muscle myoblasts and myotubes via differential antibody validation.
Cell Culture
Cell Isolation
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
07 Nov 2023
07 Nov 2023
Historique:
received:
22
05
2023
revised:
06
08
2023
accepted:
01
09
2023
medline:
8
11
2023
pubmed:
8
11
2023
entrez:
8
11
2023
Statut:
aheadofprint
Résumé
Isolation of skeletal muscles allows for the exploration of many complex diseases. Here, we present a protocol for isolating mice skeletal muscle myoblasts and myotubes that have been differentiated through antibody validation. We describe steps for collecting and preparing murine skeletal tissue, myoblast cell maintenance, plating, and cell differentiation. We then detail procedures for cell incubation, immunostaining, slide preparation and storage, and imaging for immunofluorescence validation.
Identifiants
pubmed: 37938976
pii: S2666-1667(23)00558-0
doi: 10.1016/j.xpro.2023.102591
pmc: PMC10663959
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102591Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR000445
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD090061
Pays : United States
Organisme : NHLBI NIH HHS
ID : R25 HL106365
Pays : United States
Organisme : NCRR NIH HHS
ID : UL1 RR024975
Pays : United States
Organisme : BLRD VA
ID : I01 BX005352
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK020593
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Braz J Med Biol Res. 2021 Jan 08;54(2):e10197
pubmed: 33439932
EMBO J. 2017 Jul 14;36(14):2126-2145
pubmed: 28607005
Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1889-94
pubmed: 26831110
Int J Mol Med. 2011 Aug;28(2):161-9
pubmed: 21537829
Adv Biol (Weinh). 2023 Jun;7(6):e2200221
pubmed: 36869426
Bio Protoc. 2017 May 5;7(9):
pubmed: 28730161
Methods Mol Biol. 2023;2566:113-132
pubmed: 36152246
Am J Physiol Cell Physiol. 2000 Mar;278(3):C619-26
pubmed: 10712251
Tissue Eng Part C Methods. 2009 Dec;15(4):717-28
pubmed: 19284306
Stem Cell Res. 2018 Jul;30:122-129
pubmed: 29879622
Front Cardiovasc Med. 2019 Aug 01;6:105
pubmed: 31417912
Front Bioeng Biotechnol. 2020 Sep 23;8:578140
pubmed: 33072729
Free Radic Biol Med. 2011 Nov 1;51(9):1621-35
pubmed: 21872656
Diabetes. 2014 Jan;63(1):75-88
pubmed: 24009260
Elife. 2021 May 04;10:
pubmed: 33944779
PLoS One. 2014 Jan 08;9(1):e85436
pubmed: 24416410
J Biol Chem. 2017 Mar 31;292(13):5239-5252
pubmed: 28188284
Cells. 2021 Dec 27;11(1):
pubmed: 35011629
Rev Neurol (Paris). 2022 Oct;178(8):756-765
pubmed: 35513931
Cells. 2021 Aug 24;10(9):
pubmed: 34571826
Cell Tissue Res. 1988 May;252(2):339-48
pubmed: 2838171
Adv Biol (Weinh). 2023 Oct;7(10):e2200202
pubmed: 37140138
Exp Cell Res. 2003 Dec 10;291(2):435-50
pubmed: 14644165
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Dis Model Mech. 2020 Feb 6;13(2):
pubmed: 32066552
Trends Biochem Sci. 2023 Jun;48(6):585-586
pubmed: 36990957
FEBS Lett. 2016 Oct;590(19):3309-3322
pubmed: 27587279
Rev Bras Pesqui Med Biol. 1980 Apr;13(1-3):1-8
pubmed: 7414021
Biochemistry (Mosc). 2021 May;86(5):597-610
pubmed: 33993862
Tissue Eng Part B Rev. 2014 Oct;20(5):403-36
pubmed: 24320971
Biogerontology. 2008 Apr;9(2):67-84
pubmed: 18175203
Am J Physiol Cell Physiol. 2014 Feb 15;306(4):C385-95
pubmed: 24336652
FEBS J. 2022 May;289(10):2755-2770
pubmed: 33825342