Fabrication of chitin-glucan nanofibers: Insights into mushroom pretreatment and subsequent acidic deep eutectic solvent-based esterification.

Mushrooms contain chitin-glucan complex (CGC), a natural copolymer of chitin and glucan, and nanofibrillation enhances its applicability. Here, a novel method was used to fabricate chitin-glucan nanofibers (CGNFs) from white button mushrooms. The first stage was to pretreat the raw mushroom using hot water and alkali to remove water-soluble glucans and alkali-soluble proteins, respectively, producing a CGC amenable to nanofibrillation. The second stage was nanofibrillation via esterification using acidic deep eutectic solvents (DESs) and subsequent ultrasonication. Five choline chloride-based DESs containing mono- or dicarboxylic acid were tested for the CGC esterification. DESs with strong dicarboxylic acids expedited nanofibrillation by homogeneously dispersing the solid CGC, swelling CGC fibrils, and facilitating acidity-dependent esterification leading to steric and electrostatic repulsions. One CGNF, namely CGNF_CCMnA, was characterized: it contained chitin and glucan at an approximate ratio of 8:2 and exhibited desirable properties as nanomaterials, including small diameter (11 nm) and high colloidal (zeta potential < -30 mV above pH 5.8) and thermal stability (T

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Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.