Isolation and Enrichment of Extracellular Vesicles with Double-Positive Membrane Protein for Subsequent Biological Studies.
DNA-RNA hybridization
cancer diagnosis
double-positive membrane protein
extracellular vesicles
proximity ligation assay
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
09 Nov 2023
09 Nov 2023
Historique:
received:
08
10
2023
pubmed:
9
11
2023
medline:
9
11
2023
entrez:
9
11
2023
Statut:
aheadofprint
Résumé
The isolation and enrichment of specific extracellular vesicle (EV) subpopulations are essential in the context of precision medicine. However, the current methods predominantly rely on a single-positive marker and are susceptible to interference from soluble proteins or impurities. This limitation represents a significant obstacle to the widespread application of EVs in biological research. Herein, a novel approach that utilizes proximity ligation assay (PLA) and DNA-RNA hybridization are proposed to facilitate the binding of two proteins on the EV membrane in advance enabling the isolation and enrichment of intact EVs with double-positive membrane proteins followed by using functionalized magnetic beads for capture and enzymatic cleavage for isolated EVs release. The isolated subpopulations of EVs can be further utilized for cellular uptake studies, high-throughput small RNA sequencing, and breast cancer diagnosis. Hence, developing and implementing a specialized system for isolating and enriching a specific subpopulation of EVs can enhance basic and clinical research in this field.
Identifiants
pubmed: 37942845
doi: 10.1002/adhm.202303430
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2303430Subventions
Organisme : National Science Fund for Distinguished Young Scholars
ID : 82025024
Organisme : National Key R&D Program of China
ID : 2021YFA1300604
Organisme : Key Project of the National Natural Science Foundation of China
ID : 82230080
Organisme : National Natural Science Foundation of China
ID : 82172371
Informations de copyright
© 2023 Wiley-VCH GmbH.
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