Fruit development and ripening orchestrating the biosynthesis and regulation of Lycium barbarum polysaccharides in goji berry.

Fruit development and ripening LBPs LbRIN Lycium barbarum Pectin

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
07 Nov 2023
Historique:
received: 29 08 2023
revised: 30 10 2023
accepted: 06 11 2023
pubmed: 10 11 2023
medline: 10 11 2023
entrez: 9 11 2023
Statut: aheadofprint

Résumé

Lycium barbarum polysaccharides (LBPs) are the primary bioactive components in fruits of L. barbarum, commonly known as goji berry. Despite significant progress in understanding the chemical structures and health benefits of LBPs, the biosynthesis and regulation of LBPs in goji berry remains largely unknown. In this study, physiological indicators, including LBPs, were monitored in goji berry during fruit development and ripening (FDR), suggesting that pectin might be the major component of LBPs with increased content reaching 235.8 mg/g DW. Proteomic and transcriptomic analysis show that 6410 differentially expressed genes (DEGs) and 2052 differentially expressed proteins (DEPs) were identified with overrepresentation of flavonoids and polysaccharides-related gene ontology (GO) terms and KEGG pathways. Weighted gene co-expression network analysis (WGCNA) showed that LBPs coexpress with genes involved in pectin biosynthesis (LbGALS3, LbGATL5, LbQUA1, LbGAUT1/4/7, LbRGGAT1, LbRRT1/7, and LbRHM2), modification (LbSBT1.7), and regulation (LbAP2, LbGL2 LbTLP2, LbERF4, and LbTTG2), as well as with novel transcription factors (LbSPL9 and LbRIN homologs) and glycosyltransferases. Transgenic hairy roots overexpressing LbRIN validated that LbRIN modulate the expression of WGCNA-predicted regulators, including LbERF4, LbTTG2, and LbSPL9. These findings suggest that the biosynthesis and regulation of LBPs is conserved partially to those in Arabidopsis pectin. Taken together, this study provides valuable insights into the biosynthesis and regulation of LBPs, which can facilitate future studies on synthetic biology applications and genetic improvement of LBPs.

Identifiants

DOI: 10.1016/j.ijbiomac.2023.127970 PMID: 37944729
pubmed: 37944729
pii: S0141-8130(23)04869-9
doi: 10.1016/j.ijbiomac.2023.127970
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

127970

Informations de copyright

Copyright © 2023. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Guoqi Zheng (G)

Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in the Western, School of Life Science, Ningxia University, Yinchuan 750021, Ningxia, China. Electronic address: zheng_gq@nxu.edu.cn.

Zhiqiang Wang (Z)

College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi 341000, China; Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, South China National Botanical Garden, Guangzhou 510650, China.

Jinrong Wei (J)

Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, South China National Botanical Garden, Guangzhou 510650, China. Electronic address: weijinrong@scbg.ac.cn.

Juanhong Zhao (J)

Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in the Western, School of Life Science, Ningxia University, Yinchuan 750021, Ningxia, China.

Chen Zhang (C)

Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in the Western, School of Life Science, Ningxia University, Yinchuan 750021, Ningxia, China.

Juanjuan Mi (J)

Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in the Western, School of Life Science, Ningxia University, Yinchuan 750021, Ningxia, China.

Yuan Zong (Y)

Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai, Xining, China. Electronic address: zongyuan@nwipb.cas.cn.

Genhong Liu (G)

College of Agricultural Science, Ningxia University, Yinchuan 750021, Ningxia, China.

Ying Wang (Y)

College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi 341000, China; Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, South China National Botanical Garden, Guangzhou 510650, China. Electronic address: wangying@scib.ac.cn.

Xing Xu (X)

College of Agricultural Science, Ningxia University, Yinchuan 750021, Ningxia, China.

Shaohua Zeng (S)

College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi 341000, China; Guangdong Provincial Key Laboratory of Applied Botany, State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, South China National Botanical Garden, Guangzhou 510650, China. Electronic address: shhzeng@scbg.ac.cn.

Classifications MeSH