Flavinated SDHA underlies the change in intrinsic optical properties of oral cancers.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
09 11 2023
09 11 2023
Historique:
received:
25
11
2021
accepted:
26
10
2023
medline:
13
11
2023
pubmed:
10
11
2023
entrez:
9
11
2023
Statut:
epublish
Résumé
The molecular basis of reduced autofluorescence in oral squamous cell carcinoma (OSCC) cells relative to normal cells has been speculated to be due to lower levels of free flavin adenine dinucleotide (FAD). This speculation, along with differences in the intrinsic optical properties of extracellular collagen, lies at the foundation of the design of currently-used clinical optical detection devices. Here, we report that free FAD levels may not account for differences in autofluorescence of OSCC cells, but that the differences relate to FAD as a co-factor for flavination. Autofluorescence from a 70 kDa flavoprotein, succinate dehydrogenase A (SDHA), was found to be responsible for changes in optical properties within the FAD spectral region, with lower levels of flavinated SDHA in OSCC cells. Since flavinated SDHA is required for functional complexation with succinate dehydrogenase B (SDHB), decreased SDHB levels were observed in human OSCC tissue relative to normal tissues. Accordingly, the metabolism of OSCC cells was found to be significantly altered relative to normal cells, revealing vulnerabilities for both diagnosis and targeted therapy. Optimizing non-invasive tools based on optical and metabolic signatures of cancers will enable more precise and early diagnosis leading to improved outcomes in patients.
Identifiants
pubmed: 37945749
doi: 10.1038/s42003-023-05510-w
pii: 10.1038/s42003-023-05510-w
pmc: PMC10636189
doi:
Substances chimiques
Succinate Dehydrogenase
EC 1.3.99.1
Flavin-Adenine Dinucleotide
146-14-5
SDHA protein, human
EC 1.3.5.1
Electron Transport Complex II
EC 1.3.5.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1134Subventions
Organisme : NCI NIH HHS
ID : R01 CA182043
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. The Author(s).
Références
J Biol Chem. 2016 Oct 7;291(41):21414-21420
pubmed: 27587393
Science. 2009 Aug 28;325(5944):1139-42
pubmed: 19628817
PLoS Comput Biol. 2015 Dec 01;11(12):e1004614
pubmed: 26624011
Clin Cancer Res. 2008 Apr 15;14(8):2396-404
pubmed: 18413830
Cell Metab. 2015 Jan 6;21(1):81-94
pubmed: 25565207
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3431-6
pubmed: 19202066
Annu Rev Phys Chem. 1996;47:555-606
pubmed: 8930102
Cancer Cell. 2011 Nov 15;20(5):674-88
pubmed: 22094260
Nature. 2014 Oct 30;514(7524):628-32
pubmed: 25119024
Biochim Biophys Acta. 1985 Sep 6;841(3):237-46
pubmed: 4027266
Cancer Metastasis Rev. 2005 Jan;24(1):9-17
pubmed: 15785869
Sci Rep. 2020 Feb 27;10(1):3571
pubmed: 32107448
Oral Oncol. 2009 Apr-May;45(4-5):309-16
pubmed: 18804401
Cell Metab. 2010 Sep 8;12(3):283-94
pubmed: 20816094
Cancer Cell. 2013 Jun 10;23(6):811-25
pubmed: 23764003
J Biomed Opt. 2010 Jan-Feb;15(1):017010
pubmed: 20210484
Adv Sci (Weinh). 2023 Nov;10(31):e2304632
pubmed: 37737614
Sci Rep. 2019 May 24;9(1):7833
pubmed: 31127170
Oncotarget. 2017 May 10;8(32):53819-53828
pubmed: 28881853
Cell Death Dis. 2018 Feb 7;9(2):161
pubmed: 29415987
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Oral Surg Oral Med Oral Pathol. 1984 Apr;57(4):379-82
pubmed: 6200814
Int J Mol Sci. 2013 Jan 18;14(1):1952-63
pubmed: 23334475
Chem Commun (Camb). 2016 Nov 10;52(92):13487-13490
pubmed: 27801440
ACS Biomater Sci Eng. 2023 Feb 13;9(2):932-943
pubmed: 36634351
Nat Cell Biol. 2011 Aug 28;13(10):1272-9
pubmed: 21968997
Mol Cell Biol. 2016 Jul 29;36(16):2132-40
pubmed: 27215383
Mod Pathol. 2015 Jun;28(6):807-21
pubmed: 25720320
Annu Rev Biomed Eng. 2012;14:351-67
pubmed: 22607264
J Oral Pathol Med. 1992 Apr;21(4):160-3
pubmed: 1376363
Mitochondrion. 2011 May;11(3):437-43
pubmed: 21211574
J Clin Endocrinol Metab. 2018 Feb 1;103(2):438-445
pubmed: 29177515
Head Neck. 2016 Apr;38 Suppl 1:E2403-11
pubmed: 26613806
Oncogene. 2014 Jan 30;33(5):567-77
pubmed: 23318458
Biomark Med. 2010 Apr;4(2):241-63
pubmed: 20406068
Nat Commun. 2019 Nov 1;10(1):5011
pubmed: 31676791
Nat Metab. 2019 Jan;1(1):70-85
pubmed: 31198906
Nat Cell Biol. 2008 May;10(5):611-8
pubmed: 18391940
Oncotarget. 2016 Sep 20;7(38):61890-61904
pubmed: 27542265
Mol Metab. 2020 Mar;33:48-66
pubmed: 31395464
Oncotarget. 2015 Dec 8;6(39):41706-21
pubmed: 26527315
Oncogenesis. 2020 May 15;9(5):51
pubmed: 32415061
Cell Metab. 2015 Oct 6;22(4):590-605
pubmed: 26365176
Biochem J. 2009 Jan 1;417(1):1-13
pubmed: 19061483
Oral Oncol. 2015 Jul;51(7):643-51
pubmed: 25920765
Anal Chem. 1996 Mar 1;68(5):850-8
pubmed: 8779443
Nat Rev Immunol. 2018 May;18(5):309-324
pubmed: 29379212
J Physiol. 2021 Jan;599(1):23-37
pubmed: 33006160
J Am Dent Assoc. 2010 May;141(5):509-20
pubmed: 20436098
Front Oncol. 2019 Feb 22;9:53
pubmed: 30854332
Biomater Adv. 2023 Sep;152:213495
pubmed: 37301057
Trends Biochem Sci. 2017 Apr;42(4):312-325
pubmed: 28185716
Int J Clin Exp Pathol. 2015 Oct 01;8(10):12188-97
pubmed: 26722403
Am J Surg Pathol. 2013 Feb;37(2):234-40
pubmed: 23282968
Exp Biol Med (Maywood). 2016 Jun;241(12):1281-95
pubmed: 27022139
J Am Chem Soc. 2018 Jun 20;140(24):7716-7722
pubmed: 29792690
Clin Cancer Res. 2006 Nov 15;12(22):6716-22
pubmed: 17121891
J Clin Endocrinol Metab. 2015 Mar;100(3):E531-41
pubmed: 25494863
Cell. 2012 Apr 27;149(3):656-70
pubmed: 22541435
Nat Cell Biol. 2014 Oct;16(10):992-1003, 1-15
pubmed: 25241037
Cell Metab. 2016 Feb 9;23(2):254-63
pubmed: 26777689
Cancer Res. 2016 Mar 15;76(6):1615-26
pubmed: 26795347
Cell. 2016 Oct 6;167(2):457-470.e13
pubmed: 27667687
Cureus. 2018 Aug 6;10(8):e3111
pubmed: 30338186