Induction of ferroptosis by photodynamic therapy and enhancement of antitumor effect with ferroptosis inducers.

Ferroptosis Lipid peroxidation Photodynamic therapy System xc− Talaporfin sodium

Journal

Journal of gastroenterology
ISSN: 1435-5922
Titre abrégé: J Gastroenterol
Pays: Japan
ID NLM: 9430794

Informations de publication

Date de publication:
10 Nov 2023
Historique:
received: 07 07 2023
accepted: 19 10 2023
medline: 10 11 2023
pubmed: 10 11 2023
entrez: 10 11 2023
Statut: aheadofprint

Résumé

Photodynamic therapy (PDT) is an effective tumor treatment that involves the administration of a photosensitizer to generate cytotoxic Cell viability assay in TS-PDT-treated cells in combination with a ferroptosis inhibitor (ferrostatin-1: Fer-1) or ferroptosis inducers (imidazole ketone erastin: IKE, Ras-selective lethal 3: RSL3) was performed. Accumulation of lipid peroxidation, GPX4 antioxidant system and cystine/glutamate antiporter (system xc TS-PDT-induced cell death was partly suppressed by Fer-1 and accompanied by lipid peroxidation. TS-PDT combined with IKE or RSL3 enhanced the induction of cell death. TS-PDT inhibited cystine uptake activity via system xc This study found that the mechanism underlying TS-PDT-induced ferroptosis constitutes direct lipid peroxidation by the generated ROS, and the inhibition of system xc

Sections du résumé

BACKGROUND BACKGROUND
Photodynamic therapy (PDT) is an effective tumor treatment that involves the administration of a photosensitizer to generate cytotoxic
METHODS METHODS
Cell viability assay in TS-PDT-treated cells in combination with a ferroptosis inhibitor (ferrostatin-1: Fer-1) or ferroptosis inducers (imidazole ketone erastin: IKE, Ras-selective lethal 3: RSL3) was performed. Accumulation of lipid peroxidation, GPX4 antioxidant system and cystine/glutamate antiporter (system xc
RESULTS RESULTS
TS-PDT-induced cell death was partly suppressed by Fer-1 and accompanied by lipid peroxidation. TS-PDT combined with IKE or RSL3 enhanced the induction of cell death. TS-PDT inhibited cystine uptake activity via system xc
CONCLUSION CONCLUSIONS
This study found that the mechanism underlying TS-PDT-induced ferroptosis constitutes direct lipid peroxidation by the generated ROS, and the inhibition of system xc

Identifiants

pubmed: 37947872
doi: 10.1007/s00535-023-02054-y
pii: 10.1007/s00535-023-02054-y
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Japan Society for the Promotion of Science London
ID : 20K08361
Organisme : Japan Society for the Promotion of Science London
ID : 23K07358
Organisme : Japan Society for the Promotion of Science London
ID : 22K20862
Organisme : Japan Society for the Promotion of Science London
ID : 23K15019
Organisme : Japan Society for the Promotion of Science London
ID : 23K07421
Organisme : Toyoaki Scholarship Foundation
ID : JOSE203179
Organisme : Iketani Science and Technology Foundation
ID : JOSE204007
Organisme : Bristol-Myers Squibb Foundation
ID : JOSE202103

Informations de copyright

© 2023. Japanese Society of Gastroenterology.

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Auteurs

Yuki Kojima (Y)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Mamoru Tanaka (M)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan. mtanaka@med.nagoya-cu.ac.jp.

Makiko Sasaki (M)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Keiji Ozeki (K)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Takaya Shimura (T)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Eiji Kubota (E)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Hiromi Kataoka (H)

Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-Ku, Nagoya, 467-8601, Japan.

Classifications MeSH