Effects of cytoskeletal network mesh size on cargo transport.
Journal
The European physical journal. E, Soft matter
ISSN: 1292-895X
Titre abrégé: Eur Phys J E Soft Matter
Pays: France
ID NLM: 101126530
Informations de publication
Date de publication:
10 Nov 2023
10 Nov 2023
Historique:
received:
29
06
2023
accepted:
27
09
2023
medline:
10
11
2023
pubmed:
10
11
2023
entrez:
10
11
2023
Statut:
epublish
Résumé
Intracellular transport of cargoes in the cell is essential for the organization and functioning cells, especially those that are large and elongated. The cytoskeletal networks inside large cells can be highly complex, and this cytoskeletal organization can have impacts on the distance and trajectories of travel. Here, we experimentally created microtubule networks with varying mesh sizes and examined the ability of kinesin-driven quantum dot cargoes to traverse the network. Using the experimental data, we deduced parameters for cargo detachment at intersections and away from intersections, allowing us to create an analytical theory for the run length as a function of mesh size. We also used these parameters to perform simulations of cargoes along paths extracted from the experimental networks. We find excellent agreement between the trends in run length, displacement, and trajectory persistence length comparing the experimental and simulated trajectories.
Identifiants
pubmed: 37947921
doi: 10.1140/epje/s10189-023-00358-8
pii: 10.1140/epje/s10189-023-00358-8
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109Subventions
Organisme : Directorate for Biological Sciences
ID : 2134215
Organisme : Division of Materials Research
ID : 2118403
Organisme : Division of Mathematical Sciences
ID : 1616926
Organisme : Division of Human Resource Development
ID : 1547848
Organisme : Division of Human Resource Development
ID : 2112675
Organisme : Division of Civil, Mechanical and Manufacturing Innovation
ID : 1548571
Informations de copyright
© 2023. The Author(s), under exclusive licence to EDP Sciences, SIF and Springer-Verlag GmbH Germany, part of Springer Nature.
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