Impact of volume and expression time in an AAV-delivered channelrhodopsin.
AAV
Channelrhodopsin
EPSP
Expression time
In vivo electrophysiology
Longitudinal
Optogenetics
Volume
oChIEF
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
10 Nov 2023
10 Nov 2023
Historique:
received:
08
08
2023
accepted:
02
11
2023
medline:
13
11
2023
pubmed:
11
11
2023
entrez:
11
11
2023
Statut:
epublish
Résumé
Optogenetics has revolutionised neuroscience research, but at the same time has brought a plethora of new variables to consider when designing an experiment with AAV-based targeted gene delivery. Some concerns have been raised regarding the impact of AAV injection volume and expression time in relation to longitudinal experimental designs. In this study, we investigated the efficiency of optically evoked post-synaptic responses in connection to two variables: the volume of the injected virus and the expression time of the virus. For this purpose, we expressed the blue-shifted ChR2, oChIEF, employing a widely used AAV vector delivery strategy. We found that the volume of the injected virus has a minimal impact on the efficiency of optically-evoked postsynaptic population responses. The expression time, on the other hand, has a pronounced effect, with a gradual reduction in the population responses beyond 4 weeks of expression. We strongly advise to monitor time-dependent expression profiles when planning or conducting long-term experiments that depend on successful and stable channelrhodopsin expression.
Identifiants
pubmed: 37950268
doi: 10.1186/s13041-023-01067-1
pii: 10.1186/s13041-023-01067-1
pmc: PMC10638758
doi:
Substances chimiques
Channelrhodopsins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
77Subventions
Organisme : Lundbeck Foundation
ID : R264-2017-3189
Organisme : Danmarks Grundforskningsfond
ID : DNRF133
Informations de copyright
© 2023. The Author(s).
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