3D Printed Magneto-Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models.
fiber scaffolds
magnetic actuation
melt electrowriting
skeletal muscle
stimuli responsive biomaterials
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
10 Nov 2023
10 Nov 2023
Historique:
revised:
11
10
2023
received:
20
08
2023
medline:
11
11
2023
pubmed:
11
11
2023
entrez:
11
11
2023
Statut:
aheadofprint
Résumé
This work reports the rational design and fabrication of magneto-active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a magnetized polycaprolactone-based composite. In situ iron oxide nanoparticle deposition on oxidized graphene yields homogeneously dispersed magnetic particles with sizes above 0.5 µm and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with high magnetic content (up to 20 weight %) are obtained and processed in a solvent-free manner for the first time. MEW of magnetic composites enabled the creation of skeletal muscle-inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto-active and nonactive material, with elastic tensile deformability. External magnetic fields below 300 mT are sufficient to trigger out-of-plane reversible deformation. In vitro culture of C2C12 myoblasts on three-dimensional (3D) Matrigel/collagen/MEW scaffolds showed that microfibers guided the formation of 3D myotube architectures, and the presence of magnetic particles does not significantly affect viability or differentiation rates after 8 days. Centimeter-sized skeletal muscle constructs allowed for reversible, continued, and dynamic magneto-mechanical stimulation. Overall, these innovative microfiber scaffolds provide magnetically deformable platforms suitable for dynamic culture of skeletal muscle, offering potential for in vitro disease modeling.
Identifiants
pubmed: 37950402
doi: 10.1002/smll.202307178
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2307178Subventions
Organisme : Netherlands Organisation for Scientific Research
ID : 024.003.013
Organisme : Netherlands Organisation for Scientific Research
ID : OCENW.XS5.161
Organisme : European Union Horizon 2020 (project BRAV3)
ID : 874827
Organisme : Novo Nordisk Foundation
ID : NNF21CC0073729
Organisme : FSHD Global Research Foundation Ltd
Organisme : Stichting Singel Swim Utrecht
Organisme : European Social Fund
ID : UTAP-EXPL/NPN/0044/2021
Organisme : European Social Fund
ID : UIDB/00511/2020
Organisme : European Social Fund
ID : UIDP/00511/2020
Organisme : European Social Fund
ID : LA/P/0045/2020
Organisme : European Social Fund
ID : UIDB/04293/2020
Organisme : European Social Fund
ID : 2022-04494-PTDC
Organisme : European Regional Development Fund
ID : NORTE-01-0145-FEDER-000054
Organisme : Fundação para a Ciência e a Tecnologia
ID : CEECIND/03908/2017
Organisme : Fundação para a Ciência e a Tecnologia
ID : UTAP-EXPL/NPN/0044/2021
Organisme : Fundação para a Ciência e a Tecnologia
ID : UIDB/00511/2020
Organisme : Fundação para a Ciência e a Tecnologia
ID : UIDP/00511/2020
Organisme : Fundação para a Ciência e a Tecnologia
ID : LA/P/0045/2020
Organisme : Fundação para a Ciência e a Tecnologia
ID : UIDB/04293/2020
Organisme : Fundação para a Ciência e a Tecnologia
ID : 2022-04494-PTDC
Informations de copyright
© 2023 The Authors. Small published by Wiley-VCH GmbH.
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