Protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies.
Cancer
Immunology
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
09 Nov 2023
09 Nov 2023
Historique:
received:
19
06
2023
revised:
04
08
2023
accepted:
18
10
2023
medline:
11
11
2023
pubmed:
11
11
2023
entrez:
11
11
2023
Statut:
aheadofprint
Résumé
The transplantation model provides the opportunity to assess the relevance of a molecule of interest for tumor cell extravasation by using a respective genetically modified donor animal. Here, we present a protocol for orthotopic single-lung transplantation in mice as a tool for lung metastasis studies. We describe steps for animal preparation, lung transplantation, and tumor cell injection. We then detail procedures for the direct comparison of tumor cell spreading between the genetically modified left lung and the naive right lung parenchyma. For complete details on the use and execution of this protocol, please refer to Giannou et al. (2023).
Identifiants
pubmed: 37950863
pii: S2666-1667(23)00668-8
doi: 10.1016/j.xpro.2023.102701
pmc: PMC10679946
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102701Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
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pubmed: 32024984
Immunity. 2023 Jan 10;56(1):125-142.e12
pubmed: 36630911