Ablation of
CRISPR
electroporation
gene editing
preimplantation development
Journal
PNAS nexus
ISSN: 2752-6542
Titre abrégé: PNAS Nexus
Pays: England
ID NLM: 9918367777906676
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
18
07
2023
accepted:
11
10
2023
medline:
13
11
2023
pubmed:
13
11
2023
entrez:
13
11
2023
Statut:
epublish
Résumé
CRISPR-Cas ribonucleoproteins (RNPs) are important tools for gene editing in preimplantation embryos. However, the inefficient production of biallelic deletions in cattle zygotes has hindered mechanistic studies of gene function. In addition, the presence of maternal RNAs that support embryo development until embryonic genome activation may cause confounding phenotypes. Here, we aimed to improve the efficiency of biallelic deletions and deplete specific maternal RNAs in cattle zygotes using CRISPR-Cas editing technology. Two electroporation sessions with Cas9D10A RNPs targeting exon 1 and the promoter of
Identifiants
pubmed: 37954164
doi: 10.1093/pnasnexus/pgad343
pii: pgad343
pmc: PMC10637268
doi:
Types de publication
Journal Article
Langues
eng
Pagination
pgad343Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences.
Références
Cell. 2013 May 9;153(4):910-8
pubmed: 23643243
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2846-51
pubmed: 10077599
Cancers (Basel). 2021 Jul 22;13(15):
pubmed: 34359572
Nucleic Acids Res. 2019 Aug 22;47(14):7402-7417
pubmed: 31127293
Mol Reprod Dev. 2006 Nov;73(11):1367-79
pubmed: 16894554
Mol Neurodegener. 2015 Aug 04;10:35
pubmed: 26238861
Cell Stem Cell. 2007 Oct 11;1(4):364-6
pubmed: 18371374
Reprod Fertil Dev. 2015 Jul;27(6):880-96
pubmed: 25695370
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
Cell Res. 2015 Feb;25(2):258-61
pubmed: 25430965
Biotechniques. 1993 Sep;15(3):532-4, 536-7
pubmed: 7692896
Development. 2023 Apr 15;150(8):
pubmed: 36971487
Cancers (Basel). 2019 Aug 21;11(9):
pubmed: 31438587
BMC Genomics. 2021 Feb 12;22(1):118
pubmed: 33581720
Neurotoxicol Teratol. 2021 Jan-Feb;83:106943
pubmed: 33221301
Reproduction. 2021 Jul 08;162(2):149-160
pubmed: 34096883
Mol Reprod Dev. 2004 Feb;67(2):136-44
pubmed: 14694428
Cell Death Discov. 2019 Sep 23;5:135
pubmed: 31552142
Genet Mol Biol. 2018 Jan-Mar;41(1):167-179
pubmed: 29583154
Stem Cells Int. 2017;2017:8960236
pubmed: 29434642
Sci China Life Sci. 2022 Oct;65(10):1985-1997
pubmed: 35391627
J Pathol. 2016 Jan;238(2):247-56
pubmed: 26414877
Science. 2016 Aug 5;353(6299):aaf5573
pubmed: 27256883
Cell Mol Life Sci. 2016 Aug;73(15):2959-68
pubmed: 26817461
Commun Biol. 2022 Jan 20;5(1):71
pubmed: 35058543
PLoS One. 2016 Aug 25;11(8):e0161060
pubmed: 27561029
Methods Mol Biol. 2017;1650:113-123
pubmed: 28809017
Theory Biosci. 2012 Dec;131(4):281-5
pubmed: 22872506
Biochem Soc Trans. 2022 Oct 31;50(5):1505-1516
pubmed: 36305591
J Ind Microbiol Biotechnol. 2020 May;47(4-5):413-423
pubmed: 32367443
PLoS One. 2012;7(3):e34110
pubmed: 22479535
CRISPR J. 2021 Feb;4(1):132-146
pubmed: 33616447
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
Dev Cell. 2013 Jun 24;25(6):610-22
pubmed: 23747191
Mol Reprod Dev. 1996 Jan;43(1):62-9
pubmed: 8720114
Nat Commun. 2015 Jul 09;6:7631
pubmed: 26158551
Front Genet. 2020 Sep 07;11:570069
pubmed: 33133156
J Biol Chem. 2011 Apr 15;286(15):13404-13
pubmed: 21349843
Nat Biotechnol. 2019 Aug;37(8):907-915
pubmed: 31375807
Nat Biotechnol. 2015 Jan;33(1):102-6
pubmed: 25326897
Biotechniques. 2015 Oct 01;59(4):201-2, 204, 206-8
pubmed: 26458548
Mol Reprod Dev. 2005 Oct;72(2):182-90
pubmed: 15973686
Development. 2021 Nov 15;148(22):
pubmed: 34661235
Biol Reprod. 2000 Jun;62(6):1677-84
pubmed: 10819771
CRISPR J. 2020 Dec;3(6):523-534
pubmed: 33252243
Vet Med Sci. 2022 Jan;8(1):164-169
pubmed: 34674375
J Vis Exp. 2017 Jun 15;(124):
pubmed: 28654070
Genes (Basel). 2021 Feb 16;12(2):
pubmed: 33669396
Int J Mol Sci. 2020 May 20;21(10):
pubmed: 32443745
Cell Metab. 2014 May 6;19(5):780-94
pubmed: 24746804
Gigascience. 2021 Feb 16;10(2):
pubmed: 33590861
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2770-2775
pubmed: 29483258
Mol Cell. 2015 Feb 19;57(4):685-694
pubmed: 25699711
Mol Biol Cell. 2007 Apr;18(4):1348-58
pubmed: 17267691
Bioessays. 2021 Nov;43(11):e2100186
pubmed: 34569081
Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245
pubmed: 29762716
Biol Reprod. 2021 May 7;104(5):995-1007
pubmed: 33524138
Science. 2009 Apr 24;324(5926):522-8
pubmed: 19390049
Sci Rep. 2018 May 17;8(1):7753
pubmed: 29773834
Cell. 1998 Oct 30;95(3):379-91
pubmed: 9814708
Biol Reprod. 2019 Jul 1;101(1):177-187
pubmed: 31095680
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Biochem Biophys Res Commun. 2007 Mar 30;355(1):111-6
pubmed: 17280643
CRISPR J. 2021 Dec;4(6):836-845
pubmed: 34813372
Nature. 2017 Oct 12;550(7675):280-284
pubmed: 28976959
Nat Commun. 2018 Jul 26;9(1):2937
pubmed: 30050112
Sci Rep. 2022 Jun 24;12(1):10793
pubmed: 35750764
Anim Reprod Sci. 2023 Jan;248:107174
pubmed: 36502760
Methods Mol Biol. 2019;2006:115-129
pubmed: 31230276
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Nucleic Acids Res. 2018 Jul 6;46(12):e71
pubmed: 29584876
Nature. 2017 Oct 5;550(7674):67-73
pubmed: 28953884
Front Mol Neurosci. 2018 Jul 06;11:231
pubmed: 30034322
PLoS One. 2016 Oct 5;11(10):e0163962
pubmed: 27706213
FASEB J. 2022 Jun;36(6):e22337
pubmed: 35486003
Biol Reprod. 2019 Sep 1;101(3):579-590
pubmed: 30715134
Cell Res. 2002 Dec;12(5-6):321-9
pubmed: 12528890
Science. 2017 Nov 24;358(6366):1019-1027
pubmed: 29070703
Cell Genom. 2021 Oct 13;1(1):
pubmed: 35664829
Genome Res. 2002 Jun;12(6):996-1006
pubmed: 12045153
Front Genet. 2021 Apr 13;12:648482
pubmed: 33927751
In Vitro Cell Dev Biol Anim. 2019 Sep;55(8):598-603
pubmed: 31297696
Biotechniques. 1990 Feb;8(2):148-9
pubmed: 1690559
Nat Biotechnol. 2019 Mar;37(3):224-226
pubmed: 30809026
Cell Res. 2014 Mar;24(3):372-5
pubmed: 24481528
Nat Cell Biol. 2013 Sep;15(9):1089-97
pubmed: 23934214
Cell Res. 2016 Oct;26(10):1099-1111
pubmed: 27573176
Biol Reprod. 2004 Nov;71(5):1578-82
pubmed: 15229144
PLoS One. 2015 Mar 16;10(3):e0120501
pubmed: 25775469
Blood. 2017 Dec 14;130(24):2619-2630
pubmed: 29084772
Genome Biol. 2019 Jun 24;20(1):129
pubmed: 31234903
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Theriogenology. 2019 Jul 1;132:1-11
pubmed: 30981084
Stem Cell Reports. 2015 Jun 9;4(6):1103-11
pubmed: 26028531
Nucleic Acids Res. 2012 Jan;40(Database issue):D84-90
pubmed: 22086963
Nat Biotechnol. 2020 Jun;38(6):722-727
pubmed: 32518401
STAR Protoc. 2021 Oct 19;2(4):100895
pubmed: 34723212
Stem Cells. 2021 Dec;39(12):1766-1777
pubmed: 34520582
Sci Rep. 2019 Oct 17;9(1):14900
pubmed: 31624292
Stem Cell Res. 2013 Jan;10(1):57-66
pubmed: 23117585
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Cell Rep. 2022 Jul 19;40(3):111120
pubmed: 35858556
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242
Theriogenology. 2022 Oct 15;192:22-27
pubmed: 36037573
Springerplus. 2016 Jun 21;5(1):814
pubmed: 27390654
Anim Genet. 2020 Feb;51(1):146-147
pubmed: 31625165
Methods Mol Biol. 2017;1605:231-244
pubmed: 28456969
Genes Dev. 2002 Dec 1;16(23):2991-3003
pubmed: 12464630
Nat Protoc. 2006;1(2):581-5
pubmed: 17406285
Cell. 2013 Sep 12;154(6):1380-9
pubmed: 23992846
Mol Reprod Dev. 1998 Jun;50(2):192-9
pubmed: 9590536
Nucleic Acids Res. 2022 Jun 24;50(11):e65
pubmed: 35244715
Hum Reprod. 2022 Jul 30;37(8):1760-1773
pubmed: 35700449
Sci Rep. 2017 Dec 12;7(1):17472
pubmed: 29234093
Light Sci Appl. 2021 Nov 2;10(1):224
pubmed: 34728612
Database (Oxford). 2011 Jul 23;2011:bar030
pubmed: 21785142