Glycosylation of bacterial antigens changes epitope patterns.
ACFD
Escherichia coli
SSLE
YghJ
epitope
protein glycosylation
uropathogenic
vaccine development
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
07
2023
accepted:
21
09
2023
medline:
14
11
2023
pubmed:
13
11
2023
entrez:
13
11
2023
Statut:
epublish
Résumé
Unlike glycosylation of proteins expressed in mammalian systems, bacterial glycosylation is often neglected in the development of recombinant vaccines. Here, we compared the effects of glycosylation of YghJ, an The study identifies differences in the immune response for (non)-glycosylated antigens in rabbits and pigs and compares them to a large group of patients with UTI, which have been diagnosed as positive for various bacterial pathogens. We identified glycosylation-specific peptide epitopes, a large immunological similarity between different UTI pathogens, and a broad peptide epitope pattern in patients and animals, which could result in a variable response in patients upon vaccination. This epitope analysis indicates that the vaccination of rabbits and pigs raises antibodies that translate well into the human immune system. This study underlines the importance of glycosylation in bacterial vaccines and provides detailed immune diagnostic methods to understand individual immune responses to vaccines.
Identifiants
pubmed: 37954588
doi: 10.3389/fimmu.2023.1258136
pmc: PMC10637626
doi:
Substances chimiques
Epitopes
0
Antigens, Bacterial
0
Peptides
0
YghJ protein, E coli
EC 3.4.-
Escherichia coli Proteins
0
Metalloproteases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1258136Informations de copyright
Copyright © 2023 Kern, Delaroque, Boysen, Puder, Wendt, Kölsch, Ehrentreich-Förster, Stærk, Andersen, Andersen, Lund and Szardenings.
Déclaration de conflit d'intérêts
Author AB was employed by the company GlyProVac ApS. Authors KK, MP, and MS were employed by the company Epitopic. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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