Optogenetic Microwell Array Screening System: A High-Throughput Engineering Platform for Genetically Encoded Fluorescent Indicators.

Animals Optogenetics Dopamine Analgesics, Opioid Green Fluorescent Proteins / chemistry Fluorescent Dyes / metabolism Mammals / genetics
GPCR dopamine fluorescent biosensor high throughput screening opioids protein engineering

Journal

ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031

Informations de publication

Date de publication:
24 11 2023
Historique:
pmc-release: 13 11 2024
medline: 27 11 2023
pubmed: 13 11 2023
entrez: 13 11 2023
Statut: ppublish

Résumé

Genetically encoded fluorescent indicators (GEFIs) are protein-based optogenetic tools that change their fluorescence intensity when binding specific ligands in cells and tissues. GEFI encoding DNA can be expressed in cell subtypes while monitoring cellular physiological responses. However, engineering GEFIs with physiological sensitivity and pharmacological specificity often requires iterative optimization through trial-and-error mutagenesis while assessing their biophysical function

Identifiants

DOI: 10.1021/acssensors.3c01573 PMID: 37956352 PMC: PMC10683761
pubmed: 37956352
doi: 10.1021/acssensors.3c01573
pmc: PMC10683761
doi:

Substances chimiques

Dopamine VTD58H1Z2X
Analgesics, Opioid 0
Green Fluorescent Proteins 147336-22-9
Fluorescent Dyes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

4233-4244

Subventions

Organisme : NIDA NIH HHS
ID : R00 DA049862
Pays : United States
Organisme : NIDA NIH HHS
ID : P30 DA048736
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG010461
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM109110
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1 MH130391
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM139850
Pays : United States
Organisme : NIDA NIH HHS
ID : R21 DA051193
Pays : United States
Organisme : NINDS NIH HHS
ID : U01 NS128537
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM142886
Pays : United States
Organisme : NIDA NIH HHS
ID : K99 DA049862
Pays : United States

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Auteurs

Michael Rappleye (M)

Department of Bioengineering, University of Washington, 850 Republican Street, Seattle, Washington 98105, United States.
Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.
ORCID: 0000-0002-2885-3406

Sarah J Wait (SJ)

Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.
Molecular Engineering and Sciences Institute, University of Washington, 3946 W Stevens Way NE, Seattle, Washington 98195, United States.
ORCID: 0000-0001-5660-2391

Justin Daho Lee (JD)

Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.
Molecular Engineering and Sciences Institute, University of Washington, 3946 W Stevens Way NE, Seattle, Washington 98195, United States.
ORCID: 0000-0002-0284-2389

Jamison C Siebart (JC)

The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Dr NW, Atlanta, Georgia 30332, United States.
ORCID: 0000-0002-0424-7487

Lily Torp (L)

Department of Bioengineering, University of Washington, 850 Republican Street, Seattle, Washington 98105, United States.
Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.

Netta Smith (N)

Department of Bioengineering, University of Washington, 850 Republican Street, Seattle, Washington 98105, United States.
Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.

Jeanot Muster (J)

Department of Bioengineering, University of Washington, 850 Republican Street, Seattle, Washington 98105, United States.
Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.
ORCID: 0000-0002-1972-6851

Kenneth A Matreyek (KA)

Department of Pathology, Case Western Reserve University School of Medicine, 2103 Cornell Road, Wolstein Research Building, Cleveland, Ohio 44106, United States.
ORCID: 0000-0001-9149-551X

Douglas M Fowler (DM)

Department of Genome Sciences, Foege Building S-250, 3720 15th Ave NE, Seattle, Washington 98195-5065, United States.
ORCID: 0000-0001-7614-1713

Andre Berndt (A)

Department of Bioengineering, University of Washington, 850 Republican Street, Seattle, Washington 98105, United States.
Institute of Stem Cell and Regenerative Medicine, University of Washington, 850 Republican Street, Seattle, Washington 98109, United States.
ORCID: 0000-0003-4758-5598

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Optogénétique › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Catéchols › Catécholamines › Dopamine › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents du système nerveux central › Dépresseurs du système nerveux central › Stupéfiants › Analgésiques morphiniques › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Optogenetic Microwell Array Screening System:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Optogenetic Microwell Array Screening System:...