In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis.
Colchicine
Phenotypic variation
Populus hopeiensis
Stomata
Tetraploid
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
13 Nov 2023
13 Nov 2023
Historique:
received:
15
05
2023
accepted:
02
11
2023
medline:
15
11
2023
pubmed:
14
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
Artificial induction of polyploidy is the most common and effective way to improve the biological properties of Populus and develop new varieties of this tree. In this study, in order to confirm and expand earlier findings, we established a protocol using colchicine and based on an efficient shoot regeneration system of leaf blades to induce tetraploidy in vitro in three genotypes from diploid Populus hopeiensis. The stomatal characteristics, leaf blade size, and growth were evaluated for diploids and tetraploids of three genotypes. We found that genotype, preculture duration, colchicine concentration, and colchicine exposure time had highly significant effects on the tetraploid induction rate. The optimal protocol for inducing tetraploidy in P. hopeiensis was to preculture leaf blades for 7 days and then treat them for 4 days with 40 mg/L colchicine. The tetraploid induction rates of genotypes BT1, BT3, and BT8 were 21.2, 11.4 and 16.7%, respectively. A total of 136 tetraploids were identified by flow cytometry analysis and somatic chromosome counting. The stomatal length, width, and density of leaf blades significantly differed between diploid and tetraploid plants. Compared with their diploid counterparts, the tetraploids produced larger leaf blades and had a slower growth rate. Our findings further document the modified morphological characteristics of P. hopeiensis following whole-genome duplication (e.g., induced tetraploidy). We established a protocol for in vitro induction of tetraploidy from diploid leaf blades treated with colchicine, which can be applied to different genotypes of P. hopeiensis.
Sections du résumé
BACKGROUND
BACKGROUND
Artificial induction of polyploidy is the most common and effective way to improve the biological properties of Populus and develop new varieties of this tree. In this study, in order to confirm and expand earlier findings, we established a protocol using colchicine and based on an efficient shoot regeneration system of leaf blades to induce tetraploidy in vitro in three genotypes from diploid Populus hopeiensis. The stomatal characteristics, leaf blade size, and growth were evaluated for diploids and tetraploids of three genotypes.
RESULTS
RESULTS
We found that genotype, preculture duration, colchicine concentration, and colchicine exposure time had highly significant effects on the tetraploid induction rate. The optimal protocol for inducing tetraploidy in P. hopeiensis was to preculture leaf blades for 7 days and then treat them for 4 days with 40 mg/L colchicine. The tetraploid induction rates of genotypes BT1, BT3, and BT8 were 21.2, 11.4 and 16.7%, respectively. A total of 136 tetraploids were identified by flow cytometry analysis and somatic chromosome counting. The stomatal length, width, and density of leaf blades significantly differed between diploid and tetraploid plants. Compared with their diploid counterparts, the tetraploids produced larger leaf blades and had a slower growth rate. Our findings further document the modified morphological characteristics of P. hopeiensis following whole-genome duplication (e.g., induced tetraploidy).
CONCLUSIONS
CONCLUSIONS
We established a protocol for in vitro induction of tetraploidy from diploid leaf blades treated with colchicine, which can be applied to different genotypes of P. hopeiensis.
Identifiants
pubmed: 37957587
doi: 10.1186/s12870-023-04578-0
pii: 10.1186/s12870-023-04578-0
pmc: PMC10641996
doi:
Substances chimiques
Colchicine
SML2Y3J35T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
557Subventions
Organisme : National Science Foundation of China
ID : 31570646
Informations de copyright
© 2023. The Author(s).
Références
Science. 2008 Apr 25;320(5875):481-3
pubmed: 18436776
Theor Appl Genet. 1987 Feb;73(4):589-94
pubmed: 24241118
J Biomed Biotechnol. 2009;2009:343485
pubmed: 19696915
Nat Rev Genet. 2017 Jul;18(7):411-424
pubmed: 28502977
Plant Cell Rep. 2005 Dec;24(11):671-6
pubmed: 16094528
Plant Cell Rep. 2017 Feb;36(2):313-326
pubmed: 27858216
Plants (Basel). 2019 Jun 28;8(7):
pubmed: 31261798
Science. 2006 Sep 15;313(5793):1596-604
pubmed: 16973872
Front Plant Sci. 2015 May 19;6:330
pubmed: 26042130
Mol Biol Evol. 2021 May 19;38(6):2513-2519
pubmed: 33585937
BMC Plant Biol. 2021 Sep 6;21(1):405
pubmed: 34488640
Cell Res. 2014 Oct;24(10):1274-7
pubmed: 24980958
Front Plant Sci. 2018 Mar 20;9:354
pubmed: 29616065
Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E7022-9
pubmed: 26621743
Plant Physiol Biochem. 2019 Oct;143:154-164
pubmed: 31505448
PLoS Biol. 2008 Jul 15;6(7):e174
pubmed: 18630989
J Plant Physiol. 2010 Jan 15;167(2):88-94
pubmed: 19692145
BMC Plant Biol. 2022 Apr 6;22(1):176
pubmed: 35387617
Mol Biol Evol. 2015 Sep;32(9):2351-66
pubmed: 25976351
Front Plant Sci. 2020 Mar 17;11:295
pubmed: 32256514
Hereditas. 1945;31(3-4):411-40
pubmed: 21021077
Plant Cell Rep. 2007 Nov;26(11):1977-84
pubmed: 17641861
Plant Cell Rep. 2011 Sep;30(9):1771-8
pubmed: 21750904
Int J Mol Sci. 2023 Mar 18;24(6):
pubmed: 36982881
Cell Biosci. 2021 Jun 30;11(1):119
pubmed: 34193297
PeerJ. 2021 Oct 27;9:e12399
pubmed: 34760387