The Improvement of Fluorescence In Situ Hybridization Technique Based on Explorations of Symbionts in Cicadas.
heat shock
helpers
inaccessibility
oligonucleotide probes
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
31 Oct 2023
31 Oct 2023
Historique:
received:
27
09
2023
revised:
28
10
2023
accepted:
30
10
2023
medline:
15
11
2023
pubmed:
14
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is widely used for the identification of microbes in complex samples, but it suffers from some limitations resulting in the weak or even absence of fluorescence signals of microbe(s), which may lead to the underestimation or misunderstanding of a microbial community. Herein, we explored symbionts in the bacteriomes and fat bodies of cicadas using modified FISH, aiming to improve this technique. We initially revealed that the probes of
Identifiants
pubmed: 37958818
pii: ijms242115838
doi: 10.3390/ijms242115838
pmc: PMC10650757
pii:
doi:
Substances chimiques
RNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 32270496
Organisme : National Natural Science Foundation of China
ID : 32070476
Références
Cell. 2014 Sep 11;158(6):1270-1280
pubmed: 25175626
Curr Microbiol. 2021 May;78(5):1778-1791
pubmed: 33704532
Appl Environ Microbiol. 2005 Dec;71(12):8802-10
pubmed: 16332876
Curr Protoc. 2023 Feb;3(2):e661
pubmed: 36779804
Nat Rev Genet. 2022 Jan;23(1):23-39
pubmed: 34389828
Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15394-9
pubmed: 19706397
Environ Microbiol. 2008 Oct;10(10):2894-8
pubmed: 18647333
Appl Environ Microbiol. 1998 Dec;64(12):4973-82
pubmed: 9835591
Genes (Basel). 2020 Mar 19;11(3):
pubmed: 32204543
J Vis Exp. 2012 Sep 17;(67):e4215
pubmed: 23007640
Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E226-E235
pubmed: 29279407
Appl Environ Microbiol. 2000 Aug;66(8):3603-7
pubmed: 10919826
Environ Microbiol. 2021 Nov;23(11):6603-6621
pubmed: 34390615
mBio. 2018 Mar 20;9(2):
pubmed: 29559567
Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):E5970-E5979
pubmed: 29891654
Nat Protoc. 2012 Jul 26;7(8):1534-50
pubmed: 22836135
Methods Mol Biol. 2021;2231:203-224
pubmed: 33289895
J Microbiol Methods. 2000 Jul;41(2):85-112
pubmed: 10991623
Nat Rev Microbiol. 2008 May;6(5):339-48
pubmed: 18414500
Nucleic Acids Res. 2016 Jan 4;44(D1):D586-9
pubmed: 26586809
Nucleic Acids Res. 2003 Jan 1;31(1):514-6
pubmed: 12520066
Appl Environ Microbiol. 2020 Jun 2;86(12):
pubmed: 32276978
mBio. 2018 Nov 13;9(6):
pubmed: 30425149
Trends Biotechnol. 2010 Mar;28(3):111-6
pubmed: 20005589
mBio. 2021 Aug 31;12(4):e0122821
pubmed: 34465022
Int J Mol Sci. 2023 Jan 26;24(3):
pubmed: 36768757
Proc Biol Sci. 2016 Jun 29;283(1833):
pubmed: 27358364