Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 Nov 2023
14 Nov 2023
Historique:
received:
27
10
2022
accepted:
19
10
2023
medline:
15
11
2023
pubmed:
15
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation of most of the alleles at wheat stem rust resistance gene locus SR9. The seven previously reported resistance alleles (Sr9a, Sr9b, Sr9d, Sr9e, Sr9f, Sr9g, and Sr9h) are characterised using a synergistic strategy. Loss-of-function mutants and/or transgenic complementation are used to confirm Sr9b, two haplotypes of Sr9e (Sr9e_h1 and Sr9e_h2), Sr9g, and Sr9h. Each allele encodes a highly related nucleotide-binding site leucine-rich repeat (NB-LRR) type immune receptor, containing an unusual long LRR domain, that confers resistance to a unique spectrum of isolates of the wheat stem rust pathogen. The only SR9 protein effective against stem rust pathogen race TTKSK (Ug99), SR9H, differs from SR9B by a single amino acid. SR9B and SR9G resistance proteins are also distinguished by only a single amino acid. The SR9 allelic series found in the B subgenome are orthologs of wheat stem rust resistance gene Sr21 located in the A subgenome with around 85% identity in protein sequences. Together, our results show that functional diversification of allelic variants at the SR9 locus involves single and multiple amino acid changes that recognize isolates of wheat stem rust.
Identifiants
pubmed: 37963867
doi: 10.1038/s41467-023-42747-9
pii: 10.1038/s41467-023-42747-9
pmc: PMC10645757
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7354Informations de copyright
© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
Références
Theor Appl Genet. 2014 Aug;127(8):1681-8
pubmed: 24913360
Plant Dis. 2012 May;96(5):623-628
pubmed: 30727519
Bioinformatics. 2019 Aug 15;35(16):2790-2795
pubmed: 30601942
Science. 2009 Mar 6;323(5919):1360-3
pubmed: 19229000
Theor Appl Genet. 2019 Jan;132(1):125-135
pubmed: 30327843
Nat Biotechnol. 2016 Jun;34(6):652-5
pubmed: 27111722
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Nat Commun. 2021 Jun 7;12(1):3378
pubmed: 34099713
PLoS Genet. 2018 Apr 3;14(4):e1007287
pubmed: 29614079
Plant Physiol. 2020 Jun;183(2):468-482
pubmed: 32184345
Nature. 2022 Oct;610(7932):532-539
pubmed: 36163289
Proc Natl Acad Sci U S A. 2016 Nov 8;113(45):12856-12861
pubmed: 27791121
Genes (Basel). 2020 Nov 26;11(12):
pubmed: 33256067
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9483-E9492
pubmed: 29078294
Nat Genet. 2015 Dec;47(12):1494-8
pubmed: 26551671
Nat Commun. 2022 Mar 25;13(1):1607
pubmed: 35338132
Cell. 2021 Jun 24;184(13):3528-3541.e12
pubmed: 33984278
Methods Mol Biol. 2017;1659:207-213
pubmed: 28856653
Science. 2019 Apr 5;364(6435):
pubmed: 30948527
Nat Plants. 2018 Sep;4(9):662-668
pubmed: 30150615
Nature. 2020 Dec;588(7837):277-283
pubmed: 33239791
Plant Physiol. 2013 Nov;163(3):1433-45
pubmed: 24085801
Plant Cell. 2015 Oct;27(10):2991-3012
pubmed: 26452600
Plant J. 2021 Jun;106(6):1674-1691
pubmed: 33825238
Science. 2019 Apr 5;364(6435):
pubmed: 30948526
Nat Biotechnol. 2019 Feb;37(2):139-143
pubmed: 30718880
Science. 2017 Dec 22;358(6370):1607-1610
pubmed: 29269475
Genetics. 2004 Mar;166(3):1517-27
pubmed: 15082565
Genome Res. 1998 Nov;8(11):1113-30
pubmed: 9847076
Gigascience. 2021 Feb 16;10(2):
pubmed: 33590861
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Methods Mol Biol. 2017;1659:215-229
pubmed: 28856654
Science. 2020 Dec 4;370(6521):
pubmed: 33273071
Science. 2013 Aug 16;341(6147):783-786
pubmed: 23811222
Annu Rev Phytopathol. 2007;45:289-306
pubmed: 17430087
Plant Dis. 2019 Sep;103(9):2337-2344
pubmed: 31306087
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9519-24
pubmed: 19470492
Theor Appl Genet. 2010 Jun;121(1):65-9
pubmed: 20195568
Plant Physiol. 2005 Oct;139(2):885-95
pubmed: 16183849
Genetics. 2004 Aug;167(4):1939-47
pubmed: 15342531
Science. 2017 Dec 22;358(6370):1604-1606
pubmed: 29269474
Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):E913-E921
pubmed: 28096351
Plant Dis. 2011 Jun;95(6):762-766
pubmed: 30731910
Science. 2013 Aug 16;341(6147):786-8
pubmed: 23811228
Front Plant Sci. 2020 May 26;11:678
pubmed: 32528511
Plant J. 2012 Dec;72(6):894-907
pubmed: 22805093
Methods Mol Biol. 2015;1223:189-98
pubmed: 25300841
Front Plant Sci. 2021 Oct 15;12:751398
pubmed: 34721479
G3 (Bethesda). 2021 Aug 7;11(8):
pubmed: 34849816
Nat Plants. 2021 Sep;7(9):1220-1228
pubmed: 34294906
Sci Adv. 2022 Sep 9;8(36):eabq5108
pubmed: 36083908
Nat Genet. 2023 Jun;55(6):921-926
pubmed: 37217714
New Phytol. 2020 Jan;225(2):948-959
pubmed: 31487050
Mol Biol Evol. 2013 May;30(5):1229-35
pubmed: 23486614
Science. 2020 Dec 4;370(6521):
pubmed: 33273074
Plant Biotechnol J. 2021 Jun;19(6):1206-1215
pubmed: 33415836
Nat Plants. 2015 Nov 30;1:15186
pubmed: 27251721