Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 Nov 2023
14 Nov 2023
Historique:
received:
23
01
2023
accepted:
03
11
2023
medline:
15
11
2023
pubmed:
15
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
Production of secondary metabolites is controlled by a complicated regulatory network in eukaryotic cells. Several layers of regulators are involved in this process, ranging from pathway-specific regulation, to epigenetic control, to global regulation. Here, we discover that interaction of an RNA-binding protein CsdA with a regulator RsdA coordinates fungal secondary metabolism. Employing a genetic deletion approach and transcriptome analysis as well as metabolomics analysis, we reveal that CsdA and RsdA synergistically regulate fungal secondary metabolism comprehensively. Mechanistically, comprehensive genetic and biochemical studies prove that RsdA and CsdA co-localize in the nucleus and physically interact to achieve their functions. In particular, we demonstrate that CsdA mediates rsdA expression by binding specific motif "GUCGGUAU" of its pre-mRNA at a post-transcriptional level. We thus uncover a mechanism in which RNA-binding protein physically interacts with, and controls the expression level of, the RsdA to coordinate fungal secondary metabolism.
Identifiants
pubmed: 37963872
doi: 10.1038/s41467-023-43205-2
pii: 10.1038/s41467-023-43205-2
pmc: PMC10645843
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7351Informations de copyright
© 2023. The Author(s).
Références
Environ Microbiol. 2019 Jan;21(1):416-426
pubmed: 30421486
ACS Chem Biol. 2018 Mar 16;13(3):703-711
pubmed: 29384350
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Fungi (Basel). 2021 Sep 28;7(10):
pubmed: 34682231
Nat Prod Rep. 2012 Mar;29(3):351-71
pubmed: 22228366
J Mol Biol. 2016 Feb 22;428(4):720-725
pubmed: 26410586
Eukaryot Cell. 2004 Apr;3(2):527-35
pubmed: 15075281
Eukaryot Cell. 2008 Oct;7(10):1831-46
pubmed: 18689524
Int J Mol Sci. 2021 Aug 13;22(16):
pubmed: 34445420
Annu Rev Genet. 2016 Nov 23;50:371-392
pubmed: 27732794
Microbiol Spectr. 2021 Oct 31;9(2):e0100021
pubmed: 34523975
BMC Genomics. 2018 Mar 15;19(1):200
pubmed: 29703136
Nature. 2020 Jul;583(7818):711-719
pubmed: 32728246
Mol Microbiol. 2017 Aug;105(3):469-483
pubmed: 28517364
Nat Commun. 2022 Oct 26;13(1):6361
pubmed: 36289208
Mol Plant Pathol. 2018 Dec;19(12):2635-2650
pubmed: 30047230
PLoS Pathog. 2020 Jul 15;16(7):e1008645
pubmed: 32667960
PLoS Pathog. 2011 Dec;7(12):e1002441
pubmed: 22194688
Nat Prod Commun. 2009 Nov;4(11):1505-10
pubmed: 19967983
J Nat Prod. 2020 Mar 27;83(3):770-803
pubmed: 32162523
Curr Biol. 2015 Jan 5;25(1):29-37
pubmed: 25532893
Theranostics. 2019 May 31;9(14):4101-4114
pubmed: 31281534
Org Lett. 2016 Apr 15;18(8):1832-5
pubmed: 27015125
BMC Genomics. 2015 Jan 27;16:28
pubmed: 25623211
Semin Cell Dev Biol. 2016 Sep;57:120-127
pubmed: 27021166
Sci Adv. 2022 Apr 29;8(17):eabo6094
pubmed: 35476435
Mol Microbiol. 2012 Mar;83(5):1024-34
pubmed: 22283524
Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5581-6
pubmed: 19304800
Science. 1999 May 21;284(5418):1368-72
pubmed: 10334994
J Antimicrob Chemother. 2013 Jul;68(7):1486-96
pubmed: 23580559
Eukaryot Cell. 2007 Sep;6(9):1656-64
pubmed: 17616629
Nat Chem Biol. 2009 Jul;5(7):462-4
pubmed: 19448638
J Fungi (Basel). 2021 Dec 31;8(1):
pubmed: 35049977
Nat Commun. 2021 May 11;12(1):2661
pubmed: 33976182
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Science. 2008 Jun 13;320(5882):1504-6
pubmed: 18556559
J Theor Biol. 2013 Jun 7;326:11-20
pubmed: 23458440
Nucleic Acids Res. 2015 May 26;43(10):e63
pubmed: 25712091
Nat Prod Rep. 2020 Jan 29;37(1):6-16
pubmed: 31033969
Org Biomol Chem. 2018 Jul 11;16(27):4973-4976
pubmed: 29947411
FEMS Microbiol Rev. 2019 Nov 1;43(6):591-607
pubmed: 31301226
Curr Opin Struct Biol. 2008 Jun;18(3):290-8
pubmed: 18515081
Cancers (Basel). 2020 Aug 08;12(8):
pubmed: 32784494
J Microbiol. 2011 Jun;49(3):329-39
pubmed: 21717315
PLoS One. 2016 May 24;11(5):e0156132
pubmed: 27218464
Angew Chem Int Ed Engl. 2020 Aug 3;59(32):13537-13541
pubmed: 32343875
Nat Rev Mol Cell Biol. 2014 Feb;15(2):108-21
pubmed: 24452469
Nat Rev Microbiol. 2019 Mar;17(3):167-180
pubmed: 30531948
Toxins (Basel). 2020 Feb 28;12(3):
pubmed: 32121226
Methods Mol Biol. 2012;944:163-74
pubmed: 23065615
Cell. 2021 Sep 30;184(20):5215-5229.e17
pubmed: 34559986
Cell. 2014 Jul 17;158(2):412-421
pubmed: 25036635
Nat Methods. 2008 Jul;5(7):621-8
pubmed: 18516045
Nucleic Acids Res. 2022 Sep 23;50(17):9797-9813
pubmed: 36095118
Nat Rev Mol Cell Biol. 2017 Nov;18(11):655-670
pubmed: 28951565
PLoS One. 2017 Apr 17;12(4):e0175471
pubmed: 28414767
Proc Natl Acad Sci U S A. 2021 May 25;118(21):
pubmed: 34016748
Nat Commun. 2018 Aug 30;9(1):3524
pubmed: 30166526
Cell. 2015 Dec 3;163(6):1297-300
pubmed: 26638061
Mol Microbiol. 2019 Aug;112(2):649-666
pubmed: 31116900
mBio. 2017 Sep 5;8(5):
pubmed: 28874473
Sci Adv. 2018 Apr 11;4(4):eaar5459
pubmed: 29651464
Nat Rev Microbiol. 2013 Jan;11(1):21-32
pubmed: 23178386
Nat Prod Rep. 2010 Jan;27(1):11-22
pubmed: 20024091
Nature. 2006 Oct 19;443(7113):818-22
pubmed: 17051209
Biotechnol Adv. 2021 Dec;53:107773
pubmed: 34022328
Appl Environ Microbiol. 1995 Jul;61(7):2665-73
pubmed: 7618880