Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 Nov 2023
14 Nov 2023
Historique:
received:
29
04
2022
accepted:
29
09
2023
medline:
15
11
2023
pubmed:
15
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world.
Identifiants
pubmed: 37963891
doi: 10.1038/s41467-023-42347-7
pii: 10.1038/s41467-023-42347-7
pmc: PMC10645962
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7160Subventions
Organisme : National Research Foundation of Korea (NRF)
ID : NRF-2022R1A2B5B01002343
Organisme : National Research Foundation of Korea (NRF)
ID : 2018K1A4A3A01064272
Organisme : Institute for Basic Science (IBS)
ID : IBS-R023-D1
Informations de copyright
© 2023. The Author(s).
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