IgE response to Aed al 13 and Aed al 14 recombinant allergens from

Allergens Culicidae Insect proteins Saliva Venom hypesentivity

Journal

The World Allergy Organization journal
ISSN: 1939-4551
Titre abrégé: World Allergy Organ J
Pays: United States
ID NLM: 101481283

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 07 07 2023
revised: 27 09 2023
accepted: 13 10 2023
medline: 15 11 2023
pubmed: 15 11 2023
entrez: 15 11 2023
Statut: epublish

Résumé

Mosquito bite is normally associated with mild allergic responses, but severe localized or systemic reactions are also possible. Reliable tools for the diagnosis of mosquito allergy are still unavailable. Here, we investigated the IgE response to 3 potential salivary allergens identified in the saliva of the tiger mosquito Serum from 55 adult individuals (28 controls and 27 allergic people), were analysed using an in-house Enzyme Linked ImmunoSorbent Assay (ELISA) against the Salivary Gland Extract (SGE) and the recombinant proteins Fifteen of the 27 (56%) individuals having hypersensitive reactions to mosquito bites had IgE serum levels recognizing SGE. Negative sera did not show detectable levels of IgE targeting the SGE from the most common sympatric mosquito Our results indicate that Aed al 13 and Aed al 14 hold the potential to be developed as tools for the diagnosis of allergy to

Sections du résumé

Background UNASSIGNED
Mosquito bite is normally associated with mild allergic responses, but severe localized or systemic reactions are also possible. Reliable tools for the diagnosis of mosquito allergy are still unavailable. Here, we investigated the IgE response to 3 potential salivary allergens identified in the saliva of the tiger mosquito
Methods UNASSIGNED
Serum from 55 adult individuals (28 controls and 27 allergic people), were analysed using an in-house Enzyme Linked ImmunoSorbent Assay (ELISA) against the Salivary Gland Extract (SGE) and the recombinant proteins
Results UNASSIGNED
Fifteen of the 27 (56%) individuals having hypersensitive reactions to mosquito bites had IgE serum levels recognizing SGE. Negative sera did not show detectable levels of IgE targeting the SGE from the most common sympatric mosquito
Conclusions UNASSIGNED
Our results indicate that Aed al 13 and Aed al 14 hold the potential to be developed as tools for the diagnosis of allergy to

Identifiants

pubmed: 37965096
doi: 10.1016/j.waojou.2023.100836
pii: S1939-4551(23)00096-0
pmc: PMC10641722
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100836

Informations de copyright

© 2023 The Author(s).

Déclaration de conflit d'intérêts

The authors report no competing interests.

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Auteurs

Irene Arnoldi (I)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy.
Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.
University School of Advanced Studies Pavia, IUSS, Pavia, 27100, Italy.

Marta Villa (M)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.

Giulia Mancini (G)

The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy.
University School of Advanced Studies Pavia, IUSS, Pavia, 27100, Italy.

Ilaria Varotto-Boccazzi (I)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.

Mona-Rita Yacoub (MR)

Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Chiara Asperti (C)

Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Ambra Mascheri (A)

Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Simone Casiraghi (S)

Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Sara Epis (S)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.

Claudio Bandi (C)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.

Lorenzo Dagna (L)

Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Federico Forneris (F)

The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy.

Paolo Gabrieli (P)

Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.

Classifications MeSH