Fungal drops: a novel approach for macro- and microscopic analyses of fungal mycelial growth.
bacterial–fungal interactions
fungal highways
mycelium observation
mycological method
quantification fractal dimension
user-friendly
Journal
microLife
ISSN: 2633-6693
Titre abrégé: Microlife
Pays: England
ID NLM: 9918227365406676
Informations de publication
Date de publication:
2023
2023
Historique:
received:
07
07
2023
revised:
11
10
2023
accepted:
17
10
2023
medline:
15
11
2023
pubmed:
15
11
2023
entrez:
15
11
2023
Statut:
epublish
Résumé
This study presents an inexpensive approach for the macro- and microscopic observation of fungal mycelial growth. The 'fungal drops' method allows to investigate the development of a mycelial network in filamentous microorganisms at the colony and hyphal scales. A heterogeneous environment is created by depositing 15-20 µl drops on a hydrophobic surface at a fixed distance. This system is akin to a two-dimensional (2D) soil-like structure in which aqueous-pockets are intermixed with air-filled pores. The fungus (spores or mycelia) is inoculated into one of the drops, from which hyphal growth and exploration take place. Hyphal structures are assessed at different scales using stereoscopic and microscopic imaging. The former allows to evaluate the local response of regions within the colony (modular behaviour), while the latter can be used for fractal dimension analyses to describe the hyphal network architecture. The method was tested with several species to underpin the transferability to multiple species. In addition, two sets of experiments were carried out to demonstrate its use in fungal biology. First, mycelial reorganization of
Identifiants
pubmed: 37965130
doi: 10.1093/femsml/uqad042
pii: uqad042
pmc: PMC10642649
doi:
Types de publication
Journal Article
Langues
eng
Pagination
uqad042Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.
Déclaration de conflit d'intérêts
None declared.
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