Novel helix loop-mediated isothermal amplification (HAMP) assay for colorimetric detection of Staphylococcus aureus in milk.
HAMP
Isothermal assay
Milk
Rapid detection
Staphylococcus aureus
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
15 Nov 2023
15 Nov 2023
Historique:
received:
08
09
2023
accepted:
12
11
2023
medline:
16
11
2023
pubmed:
15
11
2023
entrez:
15
11
2023
Statut:
epublish
Résumé
Staphylococcus aureus is an important and leading cause of foodborne diseases worldwide. Prompt detection and recall of contaminated foods are crucial to prevent untoward health consequences caused by S. aureus. Helix loop-mediated isothermal amplification (HAMP) is an exciting recent addition to the array of available isothermal-based nucleic acid amplification techniques. This study aimed to develop and evaluate a HAMP assay for detecting S. aureus in milk and milk products. The assay is completed in 75 minutes of isothermal temperature incubation (64 ˚C) and dye-based visual interpretation of results based on colour change. The specificity of the developed assay was ascertained using 27 S. aureus and 17 non S. aureus bacterial strains. The analytical sensitivity of the developed HAMP assay was 9.7 fg/µL of pure S. aureus DNA. The detection limit of the HAMP assay in milk (86 CFU/mL) was 1000x greater than the routinely used endpoint PCR (86 × 10
Identifiants
pubmed: 37966568
doi: 10.1007/s11274-023-03838-3
pii: 10.1007/s11274-023-03838-3
doi:
Substances chimiques
HAMP protein, human
0
Hepcidins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
14Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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