Impact of Omicron variant sublineage BA.2.75 on the OnSite COVID-19 Ag Rapid Test: the applicability of rapid antigen test with universal transport media.

Omicron variant SARS-CoV-2 diagnostic performance rapid antigen test

Journal

Infectious diseases (London, England)
ISSN: 2374-4243
Titre abrégé: Infect Dis (Lond)
Pays: England
ID NLM: 101650235

Informations de publication

Date de publication:
16 Nov 2023
Historique:
medline: 16 11 2023
pubmed: 16 11 2023
entrez: 16 11 2023
Statut: aheadofprint

Résumé

Rapid antigen testing (RAT) is one of the most powerful tools for SARS-CoV-2 detection. The OnSite COVID-19 Ag Rapid Test is an antigen-based, point-of-care test approved by the WHO for Emergency Use Listing. The To ensure the diagnostic performance of the study RAT during rapidly mutated Omicron variants. We independently evaluated the performance of this assay in 1098 archived samples collected in Thailand during October 2022-February 2023, which were 798 and 300 COVID-19 real-time RT-PCR positive and negative, respectively. The assay performed with 100% sensitivity and 100% specificity using a cycle threshold (Ct) of <20 for the RT-PCR. The sensitivity decreased to 88% when using Ct <30. Most of the SARS-CoV-2 found were Omicron BA.2 (99%), harboring six known These results confirmed the good performance of the study RAT for detecting Omicron variants and its appropriateness for individual diagnosis and for genomic surveillance.

Sections du résumé

BACKGROUND UNASSIGNED
Rapid antigen testing (RAT) is one of the most powerful tools for SARS-CoV-2 detection. The OnSite COVID-19 Ag Rapid Test is an antigen-based, point-of-care test approved by the WHO for Emergency Use Listing. The
OBJECTIVE UNASSIGNED
To ensure the diagnostic performance of the study RAT during rapidly mutated Omicron variants.
RESULTS UNASSIGNED
We independently evaluated the performance of this assay in 1098 archived samples collected in Thailand during October 2022-February 2023, which were 798 and 300 COVID-19 real-time RT-PCR positive and negative, respectively. The assay performed with 100% sensitivity and 100% specificity using a cycle threshold (Ct) of <20 for the RT-PCR. The sensitivity decreased to 88% when using Ct <30. Most of the SARS-CoV-2 found were Omicron BA.2 (99%), harboring six known
CONCLUSIONS UNASSIGNED
These results confirmed the good performance of the study RAT for detecting Omicron variants and its appropriateness for individual diagnosis and for genomic surveillance.

Identifiants

pubmed: 37970722
doi: 10.1080/23744235.2023.2280025
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-7

Auteurs

Pilailuk Akkapaiboon Okada (PA)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Pornlada Nuchnoi (P)

Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Center for Research Innovation and Biomedical Informatics, Bangkok, Thailand.

Kampaew Buayai (K)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Siripaporn Phuygun (S)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Nuttida Thongpramul (N)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Chotiros Plabplueng (C)

Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.

Archawin Rojanawiwat (A)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Ballang Uppapong (B)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Supakit Sirilak (S)

Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.

Classifications MeSH