Tunable force transduction through the
cell envelope
force transduction
gram-negative bacteria
outer membrane
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
21 Nov 2023
21 Nov 2023
Historique:
medline:
27
11
2023
pubmed:
17
11
2023
entrez:
16
11
2023
Statut:
ppublish
Résumé
The outer membrane (OM) of Gram-negative bacteria is not energised and so processes requiring a driving force must connect to energy-transduction systems in the inner membrane (IM). Tol (Tol-Pal) and Ton are related, proton motive force- (PMF-) coupled assemblies that stabilise the OM and import essential nutrients, respectively. Both rely on proton-harvesting IM motor (stator) complexes, which are homologues of the flagellar stator unit Mot, to transduce force to the OM through elongated IM force transducer proteins, TolA and TonB, respectively. How PMF-driven motors in the IM generate mechanical work at the OM via force transducers is unknown. Here, using cryoelectron microscopy, we report the 4.3Å structure of the
Identifiants
pubmed: 37972066
doi: 10.1073/pnas.2306707120
pmc: PMC10666116
doi:
Substances chimiques
Escherichia coli Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2306707120Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Références
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):5939-43
pubmed: 2068069
Nat Rev Microbiol. 2023 Aug;21(8):502-518
pubmed: 36828896
J Mol Biol. 2007 Mar 9;366(5):1424-36
pubmed: 17222427
J Biol Chem. 2001 Jul 20;276(29):27535-40
pubmed: 11328822
Nat Microbiol. 2016 Jun 20;1(7):16077
pubmed: 27572972
Annu Rev Microbiol. 2010;64:43-60
pubmed: 20420522
J Bacteriol. 2016 Jan 19;198(7):1013-21
pubmed: 26787763
Nature. 1997 Mar 20;386(6622):299-302
pubmed: 9069291
Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12353-8
pubmed: 16894158
Sci Adv. 2022 Nov 04;8(44):eadc9566
pubmed: 36322653
Science. 2006 Jun 2;312(5778):1396-9
pubmed: 16741124
J Mol Biol. 1990 Dec 20;216(4):883-95
pubmed: 2266560
FEMS Microbiol Rev. 2020 Jul 1;44(4):490-506
pubmed: 32472934
Annu Rev Biochem. 2009;78:649-72
pubmed: 19489730
Commun Biol. 2019 Oct 4;2:358
pubmed: 31602407
J Biol Chem. 2015 Oct 30;290(44):26675-87
pubmed: 26354441
Mol Microbiol. 1993 Dec;10(5):943-53
pubmed: 7934870
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5235-9
pubmed: 6310567
Mol Microbiol. 2007 Feb;63(4):1008-25
pubmed: 17233825
Mol Microbiol. 1993 Jul;9(1):219
pubmed: 8412667
Nat Methods. 2008 Feb;5(2):159-61
pubmed: 18176568
Mol Microbiol. 2001 Nov;42(3):795-807
pubmed: 11722743
Front Microbiol. 2022 Mar 03;13:852176
pubmed: 35308353
Infect Immun. 2001 Oct;69(10):6179-85
pubmed: 11553558
Biochemistry. 2001 Oct 30;40(43):13041-50
pubmed: 11669642
PLoS Comput Biol. 2021 Dec 29;17(12):e1009756
pubmed: 34965245
J Biol Chem. 1971 Apr 10;246(7):2147-51
pubmed: 4929287
FEBS Lett. 1986 Nov 24;208(2):211-6
pubmed: 3023135
Nat Microbiol. 2020 Dec;5(12):1553-1564
pubmed: 32929189
Mol Phylogenet Evol. 2013 Dec;69(3):674-86
pubmed: 23891663
Science. 1988 Dec 23;242(4886):1678-81
pubmed: 2849208
Cell. 2020 Oct 1;183(1):244-257.e16
pubmed: 32931735
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
J Bacteriol. 1998 Dec;180(24):6433-9
pubmed: 9851983
J Mol Biol. 2019 Aug 9;431(17):3275-3288
pubmed: 31153904
Aust J Exp Biol Med Sci. 1965 Apr;43:191-200
pubmed: 14323559
Annu Rev Microbiol. 2023 Sep 15;77:67-88
pubmed: 36944260
Mol Microbiol. 1992 Mar;6(6):735-42
pubmed: 1574003
Science. 2006 Jun 2;312(5778):1399-402
pubmed: 16741125
Science. 2013 Jun 28;340(6140):1570-4
pubmed: 23812713
J Mol Biol. 2002 May 3;318(3):787-804
pubmed: 12054823
Mol Microbiol. 1993 Apr;8(2):261-8
pubmed: 8316079
Protein Sci. 2010 Apr;19(4):625-30
pubmed: 20095050
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
pubmed: 10829079
Nat Rev Microbiol. 2022 Apr;20(4):236-248
pubmed: 34732874
Structure. 2018 Apr 3;26(4):590-598.e5
pubmed: 29576320
Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2306707120
pubmed: 37972066
Front Microbiol. 2022 Apr 07;13:852955
pubmed: 35464957
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
pubmed: 14665678
J Bacteriol. 1967 Oct;94(4):1112-23
pubmed: 4167587
J Mol Biol. 2005 Feb 4;345(5):1185-97
pubmed: 15644214
Mol Microbiol. 2002 May;44(3):695-708
pubmed: 11994151
Biometals. 2007 Jun;20(3-4):467-83
pubmed: 17225063
J Bacteriol. 1978 Mar;133(3):1467-71
pubmed: 346575
J Vis Exp. 2007;(6):253
pubmed: 18997900
Nat Commun. 2018 Mar 14;9(1):1082
pubmed: 29540681
Mol Microbiol. 2000 Nov;38(4):904-15
pubmed: 11115123
Gene. 1988 Apr 15;64(1):147-53
pubmed: 3294107
PLoS Genet. 2011 Sep;7(9):e1002268
pubmed: 21931562
J Bacteriol. 1981 Mar;145(3):1351-8
pubmed: 7009581
EMBO J. 2021 Nov 2;40(21):e108610
pubmed: 34515361
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Bacteriol. 1975 Jul;123(1):102-17
pubmed: 1095546
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12051-12056
pubmed: 29078392
Nat Microbiol. 2021 Feb;6(2):221-233
pubmed: 33432152
EMBO J. 2002 Aug 15;21(16):4207-18
pubmed: 12169623
Front Microbiol. 2022 Aug 05;13:948383
pubmed: 35992645
Nat Rev Microbiol. 2010 Dec;8(12):843-8
pubmed: 21060316
Biospectroscopy. 1999;5(3):189-98
pubmed: 10380085
J Bacteriol. 1997 Jun;179(11):3683-90
pubmed: 9171417
J Biol Chem. 2001 Mar 16;276(11):8111-7
pubmed: 11087740
Traffic. 2020 Aug;21(8):534-544
pubmed: 32415747
J Bacteriol. 2001 Jul;183(14):4110-4
pubmed: 11418549
Mol Microbiol. 1993 Apr;8(2):379-88
pubmed: 8316087
Nat Commun. 2020 Mar 11;11(1):1305
pubmed: 32161270
Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10348-53
pubmed: 18647830
EMBO J. 2009 Sep 16;28(18):2846-57
pubmed: 19696740