Characterization of reverse engineered anti-PF4 stereotypic antibodies derived from serum of VITT patients.


Journal

Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509

Informations de publication

Date de publication:
17 Nov 2023
Historique:
accepted: 31 10 2023
received: 26 05 2023
revised: 26 10 2023
medline: 17 11 2023
pubmed: 17 11 2023
entrez: 17 11 2023
Statut: aheadofprint

Résumé

This study presents a novel reverse-engineering approach to generate recombinant anti-PF4 antibodies from VITT-patient sera. These antibodies recapitulate functional effects of original serum anti-PF4 clonotypes, identify key PF4 binding residues and hold promise as valuable tools to investigate VITT etiology.

Identifiants

pubmed: 37976451
pii: 498764
doi: 10.1182/blood.2023021307
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2023 American Society of Hematology.

Auteurs

Jing Jing Wang (JJ)

Department of Immunology, Flinders Medical Centre, SA Pathology, Adelaide, SA, Australia.

Marijn van der Neut Kolfschoten (M)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Lucy Rutten (L)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Bridie Louise Armour (BL)

SA Pathology, Adelaide, SA, Australia.

Chee Wee Tan (CW)

University of Adelaide, Australia.

Tim Chataway (T)

Flinders University, Adelaide, Australia.

Rinke Bos (R)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Annemart Koornneef (A)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Pravien Abeywickrema (P)

Janssen, Spring House, Pennsylvania, United States.

Rick Kapur (R)

Sanquin Research, Amsterdam, Netherlands.

Leendert Porcelijn (L)

Sanquin Blood Supply, Amsterdam, Netherlands.

Midia Nouri Khalifa (MN)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Ava Sadi (A)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Pascale Bouchier (P)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Eleni Kourkouta (E)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Aditya Perkasa (A)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Ted Hj Kwaks (TH)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Roland Zahn (R)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Laura Solforosi (L)

Janssen Vaccines & Prevention B.V., Leiden, Netherlands.

Tom Paul Gordon (TP)

SA Pathology, Adelaide, SA, Australia.

Classifications MeSH