Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 Nov 2023
17 Nov 2023
Historique:
received:
03
04
2023
accepted:
22
09
2023
medline:
27
11
2023
pubmed:
18
11
2023
entrez:
18
11
2023
Statut:
epublish
Résumé
A novel tissue-specific functional tissue engineering scaffold for cartilage repair should have a three-dimensional structure, good biosafety and biological activity, and should be able to promote cartilage tissue regeneration. This study aimed to determine the effect of ultrasound-treated collagen/silk fibroin (Col/SF) composite scaffolds with good mechanical properties and high biological activity on cartilage repair. The characteristics of the scaffolds with different Col/SF ratios (7:3, 8:2, and 9:1) were determined by scanning electron microscopy, Fourier-transform infrared spectroscopy, and porosity, water absorption, and compression tests. In vitro evaluations revealed the biocompatibility of the Col/SF scaffolds. Results suggested that the optimal ratio of Col/SF composite scaffolds was 7:3. The Col/SF scaffolds induced adipose-derived stem cells to undergo chondrogenic differentiation under chondrogenic culture conditions. The efficiency of Col/SF scaffolds for cartilage regeneration applications was further evaluated using an in vivo model of full-thickness articular cartilage defects in New Zealand rabbits. The Col/SF scaffolds effectively promoted osteochondral regeneration as evidenced by macroscopic, histological, and immunohistochemical evaluation. The study demonstrates that ultrasound-treated Col/SF scaffolds show great potential for repairing cartilage defects.
Identifiants
pubmed: 37978248
doi: 10.1038/s41598-023-43397-z
pii: 10.1038/s41598-023-43397-z
pmc: PMC10656553
doi:
Substances chimiques
Fibroins
9007-76-5
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20168Subventions
Organisme : National Natural Science Foundation of China
ID : No., 81472139
Organisme : Beijing Municipal Health Commission
ID : Nos., BMHC-2019-9
Organisme : Beijing Municipal Health Commission
ID : Nos., BMHC-2021-6
Informations de copyright
© 2023. The Author(s).
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