Novel role of folate (vitamin B9) released by fermenting bacteria under Human Intestine like environment.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 Nov 2023
18 Nov 2023
Historique:
received:
03
07
2023
accepted:
10
11
2023
medline:
20
11
2023
pubmed:
19
11
2023
entrez:
19
11
2023
Statut:
epublish
Résumé
The anaerobic region of the gastrointestinal (GI) tract has been replicated in the anaerobic chamber of a microbial fuel cell (MFC). Electroactive biomolecules released by the facultative anaerobes (Providencia rettgeri) under anoxic conditions have been studied for their potential role for redox balance. MALDI study reveals the presence of vitamin B9 (folate), 6-methylpterin, para-aminobenzoic acid (PABA) and pteroic acid called pterin pool. ATR-FTIR studies further confirm the presence of the aromatic ring and side chains of folate, 6-methylpterin and PABA groups. The photoluminescence spectra of the pool exhibit the maximum emission at 420, 425, 440, and 445 nm when excited by 310, 325, 350, and 365 nm wavelengths (day 20 sample) highlighting the presence of tunable bands. The cyclic voltammetric studies indicate the active participation of pterin pool molecules in the transfer of electrons with redox potentials at - 0.2 V and - 0.4 V for p-aminobenzoate and pterin groups, respectively. In addition, it is observed that under prolonged conditions of continuous oxidative stress (> 20 days), quinonoid tetrahydrofolate is formed, leading to temporary storage of charge. The results of the present study may potentially be useful in designing effective therapeutic strategies for the management of various GI diseases by promoting or blocking folate receptors.
Identifiants
pubmed: 37980374
doi: 10.1038/s41598-023-47243-0
pii: 10.1038/s41598-023-47243-0
pmc: PMC10657476
doi:
Substances chimiques
Folic Acid
935E97BOY8
4-Aminobenzoic Acid
TL2TJE8QTX
Pterins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20226Subventions
Organisme : Penn State-COM (to M. Rawat).
ID : startup Grant-120000003048
Informations de copyright
© 2023. The Author(s).
Références
Photochem Photobiol. 1996 Mar;63(3):286-91
pubmed: 8881332
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:743-751
pubmed: 28482586
Plant Physiol. 2007 Mar;143(3):1101-9
pubmed: 17220358
Mol Pharm. 2018 Mar 5;15(3):994-1004
pubmed: 29397735
mSystems. 2020 Apr 28;5(2):
pubmed: 32345737
Adv Drug Deliv Rev. 2000 Mar 30;41(2):147-62
pubmed: 10699311
Biosens Bioelectron. 2020 Oct 1;165:112323
pubmed: 32729473
J Phys Chem B. 2012 Mar 15;116(10):3467-75
pubmed: 22364409
Tumour Biol. 2017 Jun;39(6):1010428317702649
pubmed: 28639913
Gut. 2015 Oct;64(10):1637-49
pubmed: 26041752
J Biol Chem. 2007 Mar 2;282(9):6609-6618
pubmed: 17172470
Rapid Commun Mass Spectrom. 2008 Jul;22(13):2029-43
pubmed: 18512845
J Photochem Photobiol B. 2001 Nov 15;64(2-3):179-84
pubmed: 11744405
Food Chem. 2011 Dec 1;129(3):1148-55
pubmed: 25212350
Vitam Horm. 2008;79:293-324
pubmed: 18804699
World J Gastroenterol. 2015 Aug 7;21(29):8787-803
pubmed: 26269668
Cells. 2020 Mar 04;9(3):
pubmed: 32143413
J Appl Microbiol. 2009 Jun;106(6):2017-23
pubmed: 19226393
Adv Healthc Mater. 2022 Aug;11(16):e2200447
pubmed: 35686484
Photochem Photobiol Sci. 2002 Jun;1(6):421-6
pubmed: 12856711
J Org Chem. 1976 Apr 30;41(9):1607-13
pubmed: 1263015
Sci Rep. 2019 Oct 3;9(1):14278
pubmed: 31582791
Methods Enzymol. 1980;66:657-60
pubmed: 7374506
Adv Exp Med Biol. 1993;338:351-4
pubmed: 8304137
Cancer Epidemiol Biomarkers Prev. 2008 Sep;17(9):2220-5
pubmed: 18768486
Anal Biochem. 1963 Jul;6:100-8
pubmed: 13995604
iScience. 2019 Mar 29;13:163-172
pubmed: 30844696
J Phys Chem B. 2014 Oct 9;118(40):11707-14
pubmed: 25243320
J Chromatogr A. 1999 Dec 9;864(1):59-67
pubmed: 10630871
Cancer Epidemiol Biomarkers Prev. 2007 Jul;16(7):1325-9
pubmed: 17626997
AMB Express. 2018 Apr 23;8(1):65
pubmed: 29687201
Polymers (Basel). 2022 May 13;14(10):
pubmed: 35631891
J Photochem Photobiol B. 2005 Jul 1;80(1):47-55
pubmed: 15963436
Indian J Surg Oncol. 2017 Dec;8(4):484-490
pubmed: 29203978
J Inorg Biochem. 2018 Jan;178:32-42
pubmed: 29035716
Spectrochim Acta A Mol Biomol Spectrosc. 2009 May;72(4):876-9
pubmed: 19162536
Food Res Int. 2017 Dec;102:435-444
pubmed: 29195970
Appl Environ Microbiol. 1978 Aug;36(2):306-13
pubmed: 697363
Biofabrication. 2019 Nov 06;12(1):015006
pubmed: 31519008
Curr Dev Nutr. 2020 Sep 25;4(10):nzaa153
pubmed: 33134792
Sci Rep. 2018 Aug 14;8(1):12138
pubmed: 30108274
Mol Cell Biochem. 2003 Nov;253(1-2):15-9
pubmed: 14619951
Biol Cell. 2019 Sep;111(9):232-244
pubmed: 31187884
Cells. 2019 Aug 09;8(8):
pubmed: 31405045
Gastroenterology. 2014 Nov;147(5):1055-63.e8
pubmed: 25046162
Hypoxia (Auckl). 2015 Dec 11;3:83-92
pubmed: 27774485
Cancers (Basel). 2020 Nov 11;12(11):
pubmed: 33187272
Nat Commun. 2016 May 11;7:11535
pubmed: 27168102
J Mass Spectrom. 2016 Mar;51(3):245-53
pubmed: 26956391
J Biol Chem. 2001 Jan 5;276(1):421-7
pubmed: 11022039
Eur J Biochem. 1988 Apr 15;173(2):345-51
pubmed: 3360013
Anal Bioanal Chem. 2017 Dec;409(30):7063-7075
pubmed: 28971257
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1273-80
pubmed: 3839676
PLoS One. 2015 Mar 24;10(3):e0119712
pubmed: 25803049
J Cancer. 2017 Jun 3;8(9):1586-1597
pubmed: 28775778
Biomolecules. 2019 Dec 19;10(1):
pubmed: 31861596
Appl Microbiol. 1969 Nov;18(5):723-7
pubmed: 5370458
Front Microbiol. 2019 Jul 10;10:1606
pubmed: 31354688
Talanta. 2019 Aug 15;201:465-473
pubmed: 31122452
J R Soc Interface. 2013 Mar 20;10(83):20121065
pubmed: 23516063
Crit Rev Food Sci Nutr. 2020;60(2):244-256
pubmed: 30501511