Quantitative live-cell imaging of Candida albicans escape from immune phagocytes.
Immunology
Microbiology
Microscopy
Model Organisms
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 Nov 2023
18 Nov 2023
Historique:
received:
21
07
2023
revised:
04
10
2023
accepted:
02
11
2023
medline:
19
11
2023
pubmed:
19
11
2023
entrez:
19
11
2023
Statut:
aheadofprint
Résumé
Population-level dynamics of host-pathogen interactions can be characterized using quantitative live-cell imaging. Here, we present a protocol for infecting macrophages with the fungal pathogen Candida albicans in vitro and quantitative live-cell imaging of immune and pathogen responses. We describe steps for detailed image analysis and provide resources for quantification of phagocytosis and pathogen escape, as well as macrophage membrane permeabilization and viability. This protocol is modifiable for applications with a range of pathogens, immune cell types, and host-pathogen mechanisms. For complete details on the use and execution of this protocol, please refer to Olivier et al.
Identifiants
pubmed: 37980567
pii: S2666-1667(23)00704-9
doi: 10.1016/j.xpro.2023.102737
pmc: PMC10694764
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102737Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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