Characterization of the congenital diaphragmatic hernia model in C57BL/6J fetal mice: a step toward lineage tracing experiments.
Animal model
C57BL/6J
Lineage tracing
Lung development
Maturation
Pulmonary hypertension
Pulmonary hypoplasia
Vascularization
Journal
Pediatric surgery international
ISSN: 1437-9813
Titre abrégé: Pediatr Surg Int
Pays: Germany
ID NLM: 8609169
Informations de publication
Date de publication:
20 Nov 2023
20 Nov 2023
Historique:
accepted:
25
10
2023
medline:
21
11
2023
pubmed:
20
11
2023
entrez:
19
11
2023
Statut:
epublish
Résumé
Lineage tracing is key to study the fate of individual cells and their progeny especially in developmental biology. To conduct these studies, we aimed to establish a reproducible model of CDH in the most commonly used genetic background strain that is C57BL/6J mice. CDH was induced in C57BL/6J dams by maternal administration of nitrofen + bisdiamine at E8.5. Fetuses from olive oil-gavaged mothers served as controls. Lungs from CDH and control fetuses were compared for (1) growth via radial airspace count (RAC), mean linear intercept (MLI) and gene expression for Fgf10, Nrp1, and Ctnnb1; (2) maturation (Pdpn, Spc, Ager, Abca3, Eln, Acta2, Pdgfra) via gene and protein expression; (3) vascularization via gene and protein expression (CD31, Vegfa, Vegfr1/2, Epas1, Enos). unpaired t-test or Mann-Whitney test. Nitrofen + bisdiamine administration resulted in 36% left-sided CDH (31% mortality). CDH fetuses had hypoplastic lungs and impaired growth (lower RAC, higher MLI, lower Fgf10, Nrp1, Ctnnb1), maturation (decreased Pdpn, Ager, Eln gene expression), and vascularization (decreased Cd31, Vegfr1/2; Epas1 and Enos). Lower protein expression was confirmed for PDPN, ELN and CD31. Modeling CDH in C57BL/6J mouse fetuses is effective in reproducing the classical CDH hallmarks. This model will be critical for lineage tracing experiments.
Identifiants
pubmed: 37981587
doi: 10.1007/s00383-023-05583-y
pii: 10.1007/s00383-023-05583-y
doi:
Substances chimiques
nitrofen
N71UYG034A
Transcription Factors
0
nitrophen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
296Subventions
Organisme : CIHR
ID : 175300
Pays : Canada
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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