The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition.
TORC1
proteostasis
ribosome-associated chaperones
stress
translation
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
11 Dec 2023
11 Dec 2023
Historique:
revised:
23
10
2023
received:
08
12
2022
accepted:
26
10
2023
pubmed:
21
11
2023
medline:
21
11
2023
entrez:
20
11
2023
Statut:
ppublish
Résumé
Protein requirements of eukaryotic cells are ensured by proteostasis, which is mediated by tight control of TORC1 activity. Upon TORC1 inhibition, protein degradation is increased and protein synthesis is reduced through inhibition of translation initiation to maintain cell viability. Here, we show that the ribosome-associated complex (RAC)/Ssb chaperone system, composed of the HSP70 chaperone Ssb and its HSP40 co-chaperone Zuo1, is required to maintain proteostasis and cell viability under TORC1 inhibition in Saccharomyces cerevisiae. In the absence of Zuo1, translation does not decrease in response to the loss of TORC1 activity. A functional interaction between Zuo1 and Ssb is required for proper translational control and proteostasis maintenance upon TORC1 inhibition. Furthermore, we have shown that the rapid degradation of eIF4G following TORC1 inhibition is mediated by autophagy and is prevented in zuo1Δ cells, contributing to decreased survival in these conditions. We found that autophagy is defective in zuo1Δ cells, which impedes eIF4G degradation upon TORC1 inhibition. Our findings identify an essential role for RAC/Ssb in regulating translation in response to changes in TORC1 signalling.
Identifiants
pubmed: 37984430
doi: 10.15252/embj.2022113240
pmc: PMC10711665
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e113240Subventions
Organisme : UKRI | Medical Research Council (MRC)
ID : MC_UU_00018/8
Informations de copyright
© 2023 The Authors. Published under the terms of the CC BY 4.0 license.
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