Kinetic and structural assessment of the reduction of human 2-Cys peroxiredoxins by thioredoxins.
catalytic cycle
hydrogen peroxide
peroxiredoxin
reaction mechanism
redox signaling
thioredoxin
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
20 Nov 2023
20 Nov 2023
Historique:
revised:
09
10
2023
received:
14
05
2023
accepted:
16
11
2023
pubmed:
21
11
2023
medline:
21
11
2023
entrez:
20
11
2023
Statut:
aheadofprint
Résumé
We have studied the reduction reactions of two cytosolic human peroxiredoxins (Prx) in their disulfide form by three thioredoxins (Trx; two human and one bacterial), with the aim of better understanding the rate and mechanism of those reactions, and their relevance in the context of the catalytic cycle of Prx. We have developed a new methodology based on stopped-flow and intrinsic fluorescence to study the bimolecular reactions, and found rate constants in the range of 10
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agencia Nacional de Investigación e Innovación
ID : FCE 2019-155969
Organisme : Comisión Sectorial de Investigación Científica
ID : Grupos 2018
Informations de copyright
© 2023 Federation of European Biochemical Societies.
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