Systematic Dissection of Sequence Features Affecting the Binding Specificity of a Pioneer Factor Reveals Binding Synergy Between FOXA1 and AP-1.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
11 Nov 2023
Historique:
pubmed: 21 11 2023
medline: 21 11 2023
entrez: 21 11 2023
Statut: epublish

Résumé

Despite the unique ability of pioneer transcription factors (PFs) to target nucleosomal sites in closed chromatin, they only bind a small fraction of their genomic motifs. The underlying mechanism of this selectivity is not well understood. Here, we design a high-throughput assay called ChIP-ISO to systematically dissect sequence features affecting the binding specificity of a classic PF, FOXA1. Combining ChIP-ISO with

Identifiants

DOI: 10.1101/2023.11.08.566246 PMID: 37986839 PMC: PMC10659273
pubmed: 37986839
doi: 10.1101/2023.11.08.566246
pmc: PMC10659273
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM127034
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM139654
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM144135
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM125592
Pays : United States

Auteurs

Cheng Xu (C)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Holly Kleinschmidt (H)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Jianyu Yang (J)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Erik Leith (E)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Jenna Johnson (J)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.

Song Tan (S)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Shaun Mahony (S)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.

Lu Bai (L)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Eukaryotic Gene Regulation, The Pennsylvania State University, University Park, PA 16802, USA.
Department of Physics, The Pennsylvania State University, University Park, PA 16802, USA.

Classifications MeSH